NeuroMab™ Mouse Anti-TFEB Monoclonal Antibody (CBP2260)

Product Overview

Description

The autophagy-lysosomal pathway is involved in the degradation of long-lived proteins. The transcription factor EB (TFEB) plays a critical role in regulating basic cellular processes, such as lysosomal biogenesis and autophagy. The subcellular localization and activity of TFEB are regulated by mechanistic target of rapamycin (mTOR)-mediated phosphorylation. TFEB is also involved in physiological processes, and it induces the intracellular clearance of pathogenic factors in a variety of diseases, such as Parkinson's and Alzheimer's, suggesting that novel therapeutic strategies could be based on the modulation of TFEB activity.

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b, κ

Clone Number

CBP2260

Applications

WB; IHC-P

Application Notes

WB (0.5-5.0 µg/mL); IHC (5-10 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1673

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

TFEB

Official Name

TFEB

Full Name

Transcription factor EB

Alternative Names

TFEB; ALPHABHLHE35; TCFEB; transcription factor EB

Gene ID

7942 (Human); 21425 (Mouse)

Uniprot ID

P19484 (Human); Q9R210 (Mouse)

References

1. Martini-Stoica H, et al. 2016. Trends Neurosci. 221-234.
2. Napolitano G, Ballabio A. 2016. J. Cell Sci. 129: 2475-81.
3. Raben N, Puertollano R. 2016. Annu. Rev. Cell Dev. Biol. 32: 255-278.

Publications