Mouse Anti-Nestin Monoclonal Antibody (CBP6703)
[CAT#: NAB2007FY1564]
Mouse Monoclonal to Nestin
- Host Species:
- Mouse
- Species Reactivity:
- Human; Rat; Mouse
- Applications:
- WB; IF; IHC
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
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Description
Nestin is a member of the class IV intermediate filament protein family expressed in neuroepithelial stem cells, which is also the origin of the name of nestin. Nestin was originally identified by producing a series of monoclonal antibodies against epitopes expressed on rat spinal cord tissue on day 15 of formalin-fixed embryos (1). One of the antibodies, called Rat 401, stains fiber distribution in the developing nervous system, but does not stain in the mature nervous system. Screening of phage expression libraries by using Rat 401 antibody, Lendahl et al. (2) The cDNA encoding the protein binding to rat 401 antibody can be isolated.
Immunogen
The recombinant construct, amino acids 317-630 of human protein was expressed in E. coli and purified from E. coli.
Species Reactivity
Human; Rat; Mouse
Clonality
Monoclonal
Host Species
Mouse
Isotype
IgG1
Clone Number
CBP6703
Applications
WB; IF; IHC
Application Notes
WB: 1:500
IF/ICC: 1:500
IF/ICC: 1:500
Product Properties
Form
Liquid
Formulation
50% PBS, 50% glycerol
Preservatives
5mM NaN3
Concentration
1 mg/ml
Shipping
Gel Pack
Storage
Store at 4°C for short term, for longer term store at -20°C
Handling Advice
The antibody solution should be gently mixed before use.
Research Use Only
For research use only
Target
Publications
Publications (0)
Neural Antibodies
Kits
For Research Use Only. Not For Clinical Use.
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