NeuroMab™ Mouse Anti-NES Monoclonal Antibody (CBP1598)

Description

Mouse anti-human Nestin antibody, clone 10C2 recognizes nestin, which is a large intermediate filament protein originally identified by (Hockfield and McKay 1985). Nestin is mainly expressed in stem cells of the developing nervous system. Terminal differentiation is related to the loss of nestin expression. In other embryonic tissues, in most glioblastoma polymorphs and many melanomas, nestin expression has also been noted.

Species Reactivity

Human; Cynomolgus monkey

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1598

Applications

IHC-P; IHC-Fr; WB

Application Notes

IHC-P (1/50-1/200); WB (1/500-1/1000)

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1378

Form

Liquid

Formulation

PBS
0.1% Sodium Azide

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

NES

Official Name

NES

Full Name

Nestin

Alternative Names

Nestin; Nbla00170

Gene ID

10763(Human); 18008(Mouse)

Uniprot ID

P48681(Human); Q6P5H2(Mouse); P21263(Rat)

1. Hockfield, S. & McKay, R.D. (1985) Identification of major cell classes in the developing mammalian nervous system. 2. Martínez-Navarrete, G.C. et al. (2008) Gradual morphogenesis of retinal neurons in the peripheral retinal margin of adult monkeys and humans 3. González-Garza, M.T. et al. (2013) Differentiation of CD133+ stem cells from amyotrophic lateral sclerosis patients into preneuron cells. 4. Aljammal, K. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades 5. Sterlacci, W. et al. (2014) Putative stem cell markers in non-small-cell lung cancer: a clinicopathologic characterization. 6. Nowak, A. et al. (2017) Nestin-positive microvessel density is an independent prognostic factor in breast cancer. 7. Arranz, L. et al. (2014) Neuropathy of haematopoietic stem cell niche is essential for myeloproliferative neoplasms. 8. Del Toro, R. et al. (2016) Nestin(+) cells direct inflammatory cell migration in atherosclerosis.