beta3 adrenoceptor Assay Cell Line

[CAT#: NCL2110P305]

Cell Types:: Other Cells

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Product Overview

Description

Cell line information: ADRB3 cAMP Maxensor cell line
Official Full Name: Adrenoceptor Beta 3
DNA Accession Number: AY487247
Host Cells: U2OS Cell Line
Resistance: G418 + Puromycin
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen

Features

The red cAMP Maxensor-ADRβ3 contains U2OS cells stably expressing the red cAMP Maxensor biosensor and adrenergic receptor Beta 3 (unlabeled). Creative Biolabs' cAMP Maxensor-ADRβ3 cell line is designed to test compounds or analyze their ability to modulate the beta 3 adrenergic receptor. When the agonist binds to ADRβ3, the G protein is activated, which in turn triggers a cellular response mediated by cAMP. This cell line has been verified by measuring the increase in cAMP in the cytosol, analyzing the increase in red fluorescence and the distribution of biosensors in the cell. The use of isoproterenol as an agonist in high-throughput screening (HTS) and high-content analysis (HCA) validates this highly reproducible assay.

Cell Types

Other Cells

Application Notes

About Red cAMP Maxensor Biosensor:
The red cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before stimulating cAMP production, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the red cAMP Maxensor biosensor, which promotes the relocation of cells in the transport of cell vesicles. In cell lines that co-express the red cAMP Maxensor biosensor and the GPCR of interest, the activity of living cells can be easily quantified by fluorescence granularity image analysis or fluorescence intensity analysis.

Research Areas

GPCR

Assay

beta3 adrenoceptor Assay

Assay Description

β-3 adrenoceptor (β3 adrenoceptor); also known as ADRB3, is a β-adrenoceptor that spans cell membranes. Creative Biolabs' beta3 adrenergic receptor test (ADRB3) allows the use of Maxensor biosensor technology to measure ADRB3 receptor activation. Beta3 adrenergic receptor signaling occurs through adenylate cyclase stimulation through the interaction of trimerized Gs protein, increased cAMP, and downstream L-type calcium channels. The cAMP-Maxensor biosensor measures receptor activation through cAMP accumulation.

Assay Characteristics

Readout: cAMP Flux

Agonist: Isoproterenol

EC50 Agonist: 2.64 x 10⁶-11 M for cAMP Flux

Type of Assay: Cell-based / Functional

Detection Method: Fluorimetry

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.