Human CHO-K1/5-HT4/Gα15 Stable Cell Line

[CAT#: NCL20120006CR] Review(5) Q&As(3)

5-HT4 Serotonin Receptor Stable Cell Line

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

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Product Overview

Description

5-HT4 is a human serotonin G protein-coupled receptor. Several studies suggested that intact 5-HT systems are important determinants of sensitivity and/or tolerance to ethanol-induce ataxia hypothermia. Attenuated response to stress and novelty and hypersensitivity to seizures in 5-HT receptor knock-out mice. Neuropsychiatric disorders such as anxiety, depression, migraine, vasospasm and epilepsy may involve different subtypes of the 5-HT receptor.

Cell Types

Other Cells

Applications

GPCR Screening

Research Areas

GPCR; Neural Signal Transduction

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen cells

Culture Medium

Ham's F12, 10% FBS, 400 μg/ml G418, 200 μg/ml Hygromycin B

Freeze Medium

45% culture medium, 45% FBS, 10% DMSO

Receptor

HTR4

Family

5-Hydroxytryptamine receptor

Strain

5-HT4/Gα15 Cell Line

Cell Purity

>95%

Shipping

Dry ice

Storage

Liquid nitrogen immediately upon delivery

Handling Advice

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Target Details

Target

5-HT4

Official Name

5-HT4

Full Name

5-hydroxytryptamine (serotonin) receptor 4

Alternative Names

5-HT4 receptor; 5-HT4; 5-hydroxytryptamine (serotonin) receptor 4; 5-HT-4; 5-HT4 receptor (two splicing variants L and S); 5-hydroxytryptamine receptor 4; serotonin receptor 4; 5 hydroxytryptamine (serotonin) receptor 4

Gene ID

3360(Human); 15562(Mouse); 25324(Rat)

Uniprot ID

Q13639(Human); P97288(Mouse); Q62758(Rat)

References

1. Yang, I., Fortin, M. C., Richardson, J. R., & Buckley, B. (2011). Fused-core silica column ultra-performance liquid chromatography-ion trap tandem mass spectrometry for determination of global DNA methylation status. Analytical biochemistry, 409(1), 138-143.

Publications

Publications (0)

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Scientific Resources

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

The cells exhibit robust expression of the 5-HT4 receptor and have consistently provided reliable data

We appreciate the stability and high transfection efficiency of this cell line, which has significantly accelerated our research progress. Additionally, the customer support provided by the supplier was exceptional, addressing all our inquiries promptly.

Excellent

We are very satisfied with the results

The cells are easy to culture and maintain, and their responsiveness in GPCR screening is outstanding. The signal-to-noise ratio is excellent, allowing us to detect even subtle changes in receptor activity.

Excellent

The cells display consistent receptor expression and functional coupling to Gα15

We have achieved reproducible results across multiple batches, highlighting the cell line's stability. The technical support team was very helpful in providing detailed protocols and troubleshooting tips.

Excellent

Enhanced our GPCR screening capabilities

The cells are robust, and their high receptor expression levels have improved the sensitivity of our assays. We have successfully identified several potential drug candidates using this cell line. The product was delivered promptly, and the documentation provided was thorough and easy to follow.

Excellent

The cells are highly responsive to 5-HT4 agonists and antagonists

The cell line's stability over long-term culture has been particularly beneficial for our high-throughput screening projects.

Q&As

How stable is the expression of the 5-HT4 receptor and Gα15 protein in this cell line?

The expression of the 5-HT4 receptor and Gα15 protein in the Human CHO-K1/5-HT4/Gα15 Stable Cell Line is highly stable. The cell line is maintained under selective pressure with appropriate antibiotics, ensuring consistent expression over multiple passages. This stability is crucial for obtaining reproducible and reliable experimental data in GPCR screening studies.

What is the doubling time of the human CHO-K1/5-HT4/Gα15 stable cell line?

The doubling time of the cell line is approximately 18-24 hours under optimal culture conditions. This growth rate may vary slightly depending on specific laboratory conditions and culture practices. Regular monitoring of cell confluence and timely passaging are recommended to maintain healthy and proliferative cultures.

What is the expected signal-to-noise ratio for calcium flux assays using this cell line?

The cell line typically exhibits a high signal-to-noise ratio in calcium flux assays due to the stable expression of the 5-HT4 receptor and Gα15 protein. This ensures robust and reliable measurements of intracellular calcium levels upon receptor activation. The exact signal-to-noise ratio may vary depending on the assay conditions and instrumentation used, but it is generally well-suited for detecting receptor-mediated calcium responses.