Human CHO-K1/AGTRL1/Gα15 Stable Cell Line

[CAT#: NCL20120027CR] Review(5) Q&As(3)

Apelin Receptor Stable Cell Line

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

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Product Overview

Description

AGTRL1 is Gi/Go-coupled GPCRs expressed in the heart, coronary artery, aorta, internal mammary artery, pulmonary artery and saphenous vein, it also expressed in lung, kidney and adrenal gland. In AGTRL1 receptor knockout mice baseline blood pressure is not changed compared to wild-type animals. Vasoconstrictor responses to angiotensin II however are significantly more pronounced in the knockout animals.

Cell Types

Other Cells

Applications

GPCR Screening

Research Areas

GPCR

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen cells

Culture Medium

Ham's F12, 10% FBS, 100 μg/ml Hygromycin B, 400 μg/ml G418

Freeze Medium

45% culture medium, 45% FBS, 10% DMSO

Receptor

AGTRL1

Family

Apelin receptor

Strain

AGTRL1/Gα15 Cell Line

Cell Purity

>95%

Shipping

Dry ice

Storage

Liquid nitrogen immediately upon delivery

Handling Advice

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Target Details

Target

AGTRL1

Official Name

AGTRL1

Alternative Names

APJ; APJ (apelin) receptor; APJ receptor; APJR; G protein-coupled receptor APJ; G-protein coupled receptor APJ; G-protein coupled receptor HG11; HG11; HG11 orphan receptor; angiotensin II receptor-like 1; angiotensin receptor-like 1; apelin receptor

Gene ID

187(Human); 23796(Mouse); 83518(Rat)

Uniprot ID

P35414(Human); Q9WV08(Mouse); Q9JHG3(Rat)

References

1. Watanabe, H., Matsushita, N., Shimizu, Y., Iikuni, S., Nakamoto, Y., Togashi, K., & Ono, M. (2019). Synthesis and characterization of a novel 18 F-labeled 2, 5-diarylnicotinamide derivative targeting orexin 2 receptor. MedChemComm, 10(12), 2126-2130.

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

An excellent tool for our GPCR screening projects

The cells exhibit robust expression and functionality of the AGTRL1 receptor, which has significantly accelerated our assay development. The cell line is highly stable, and we have observed consistent results across multiple passages.

Excellent

It has greatly improved our GPCR screening throughput

The Gα15 coupling allows for efficient detection of receptor activity, and the cells have shown excellent viability and reproducibility in our assays. This cell line is a valuable addition to our lab.

Excellent

Our lab specializes in GPCR pharmacology

The CHO-K1/AGTRL1/Gα15 cell line is easy to culture and maintain, and their response to ligands is consistent and reproducible. This has allowed us to generate high-quality data and advance our research projects more efficiently.

Excellent

The stable cell line has exceeded our expectations in every aspect

We have used this cell line for both high-throughput screening and detailed mechanistic studies, and it has performed exceptionally well in all applications.

Excellent

We have been able to generate high-quality data and accelerate our research with this cell line

As a GPCR researcher, I highly value the quality and performance of the CHO-K1/AGTRL1/Gα15 cell line. The cells exhibit reliable receptor expression and functionality, making them ideal for our screening assays. The Gα15 coupling enhances the sensitivity of our assays, allowing us to detect subtle changes in receptor activity with confidence.

Q&As

Can this cell line be adapted for use with other GPCRs, or is it specific to AGTRL1?

While the CHO-K1/AGTRL1/Gα15 stable cell line is specifically designed for AGTRL1, the underlying CHO-K1 cell line can be adapted for other GPCRs. This involves generating new stable cell lines with the desired GPCR and ensuring proper coupling with appropriate signaling proteins. If you require a different GPCR, we can assist with creating customized cell lines tailored to your specific research needs.

What are the storage and handling recommendations for this cell line?

The CHO-K1/AGTRL1/Gα15 stable cell line should be stored in liquid nitrogen for long-term storage. For short-term handling, maintain the cells at 37°C in a humidified incubator with 5% CO₂. When thawing, quickly transfer the cells to a pre-warmed culture medium and follow proper cell culture protocols to ensure the health and stability of the cell line.

What support is available if I encounter issues with this cell line during my research?

We provide comprehensive support for customers using the CHO-K1/AGTRL1/Gα15 stable cell line. Our technical support team is available to assist with any issues you may encounter, including troubleshooting experimental protocols and optimizing assay conditions. We also offer guidance on cell culture practices and can provide additional resources or advice based on your specific research needs to ensure successful outcomes.