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Creative Biolabs
Product

Human DMPK Knockout HCT116 Cell Lysate

[CAT#: NCL2008ZP548]

Human DMPK knockout cell lysate

Species:
Human
Cell Types:
HCT116 Cell

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Product Overview

Description

This is a HCT116 cell transfected lysate in which Human DMPK has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HCT116 Cell

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon1.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurodegeneration

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 10, 11
D13S317: 10, 12
D7S820: 11, 12
D16S539: 11, 13
vWA: 17, 22
TH01: 8,9
TPOX: 8, 9
CSF1PO: 7, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

DMPK

Official Name

DMPK

Full Name

myotonic dystrophy protein kinase

Alternative Names

DMPK; DM; DM1; DM1PK; DMK; MDPK; MT-PK; Dm15; dystrophia myotonica protein kinase; DM1 protein kinase

Gene ID

1760(Human); 13400(Mouse); 308405(Rat)

Uniprot ID

Q09013(Human); P54265(Mouse); D3ZYV4(Rat)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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