Human LIF Knockout HeLa Cell Line
Human LIF knockout cell line
- Species:
- Human
- Cell Types:
- Other Cells
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Description
Cell Types
Cell Location
Application Notes
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Passage Number
Knockout Validation
Research Areas
Species
Size
Formulation
Growth Pattern
STR Analysis
Biosafety Level
Tissue Source
Cell Purity
Cell Viability
Antibiotic Resistance
Mycoplasma Testing
Sterility Testing
Genetic Stability Testing
Shipping
Storage
Handling Advice
1. Thaw the vial in a 37°C water bath for about 1-2 minutes.
2. Transfer the cell suspension to a 15 mL conical tube containing pre-warmed 5 mL complete medium DMEM + 10% FBS, and rotate 125 x g for about 5 minutes at room temperature.
3. Resuspend the cell pellet with 1 mL of pre-warmed complete medium DMEM + 10% FBS, and distribute it into a 25 cm2 culture flask, which contains 10 mL of pre-warmed complete medium DMEM + 10% FBS.
4. Incubate the culture with 5% CO2 in a 37°C incubator.
5. The recommended subculture ratio is 1:4-1:6. When cells grow at 80-90% confluence and divide, the cells should be passaged.
Research Use Only
Warnings
Quality Control
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