Human SIAH1 Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP200]

Human SIAH1 knockout cell lysate

Species:: Human

Cell Types:: Other Cells

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

This is a HeLa cell transfected lysate in which Human SIAH1 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2 and 4 bp deletion in exon2.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neural Signal Transduction

Pathways

Notch Pathway

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

SIAH1

Official Name

SIAH1

Alternative Names

Siah E3 Ubiquitin Protein Ligase 1; RING-Type E3 Ubiquitin Transferase SIAH1; Seven In Absentia Homolog 1; Siah-1a; Seven In Absentia Homolog 1 (Drosophila); E3 Ubiquitin-Protein Ligase SIAH1;