iNeu™ DopaNeurons PD SNCA A53T HZ Kit, Disease Model
[CAT#: NCL-7P026]
DopaNeurons Parkinson's Disease differentiated from Human iPS cells, frozen
- Species:
- Human
- Cell Types:
- Dopaminergic Neurons
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
SNCA A53T is one of the most permeable and widely studied mutations associated with Parkinson's disease (PD). SNCA encodes the alpha-synuclein (α-syn) protein, which is mainly expressed at the presynaptic terminal of the brain The A53T mutation makes α-syn more likely to accumulate and accumulate, which is a hallmark indicator of PD pathology.
In order to generate heterozygous (HZ) A53T allelic variants that are syngeneic to iNeu® DopaNeurons, nuclease-mediated SNP changes were performed on healthy control iPS cell lines to introduce this site-specific mutation into the SNCA gene. Then, this genome-engineered iPS cell differentiates into Human midbrain floor plate dopaminergic (DA) neurons to produce iNeu DopaNeurons SNCA A53T HZ.
In order to generate heterozygous (HZ) A53T allelic variants that are syngeneic to iNeu® DopaNeurons, nuclease-mediated SNP changes were performed on healthy control iPS cell lines to introduce this site-specific mutation into the SNCA gene. Then, this genome-engineered iPS cell differentiates into Human midbrain floor plate dopaminergic (DA) neurons to produce iNeu DopaNeurons SNCA A53T HZ.
Features
Easy to implement
iNeu DopaNeurons are shipped cryopreserved with optimized media. Simply thaw and use.
Homogenous and reproducible
iNeu DopaNeurons are fully differentiated neurons, not precursors, providing biologically relevant and reproducible results.
Highly pure
iNeu DopaNeurons are>80% tyrosine hydroxylase positive (THpos) midbrain dopaminergic neurons, enabling mechanistic studies of neurodegenerative diseases.
Human cells
iNeu DopaNeurons are floor plate-derived dopaminergic neurons from Human iPS cells ensuring complete regional specification for full functionality.
iNeu DopaNeurons are shipped cryopreserved with optimized media. Simply thaw and use.
Homogenous and reproducible
iNeu DopaNeurons are fully differentiated neurons, not precursors, providing biologically relevant and reproducible results.
Highly pure
iNeu DopaNeurons are>80% tyrosine hydroxylase positive (THpos) midbrain dopaminergic neurons, enabling mechanistic studies of neurodegenerative diseases.
Human cells
iNeu DopaNeurons are floor plate-derived dopaminergic neurons from Human iPS cells ensuring complete regional specification for full functionality.
Neurological Disease Models
PD-related Cells
Cell Types
Dopaminergic Neurons
Relevant Diseases
Parkinson's Disease
Species
Human
Properties
Size
1 Vial: ≥ 1 x 10^6 cells/vial
Components
Cryopreserved MyCell DopaNeurons SNCA A53T HZ, 01279
100 ml iNeu Neural Base Medium 1
2 ml iNeu Neural Supplement B
1 ml iNeu Nervous System Supplement
100 ml iNeu Neural Base Medium 1
2 ml iNeu Neural Supplement B
1 ml iNeu Nervous System Supplement
Tissue Source
Blood
Age
50-59
Mycoplasma Testing
The cell line has been screened using the Mycoplasma Detection Kit to confirm the absence of Mycoplasma contamination.
Shipping
Dry ice.
Storage
Liquid Nitrogen
Research Use Only
For research use only
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Target Details
Target
SNCA
Donor Information
Gender
Male
Donor Age
50-59
Donor Health
Disease
Ethnicity
Caucasian
Additional Donor Information
Zygosity: Heterozygous
Genotype: SNCA (A53T) c.209G>A
Genotype: SNCA (A53T) c.209G>A
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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