NeuroMab™ Anti-CD282 Antibody(NRP-0422-P734)
A functional antibody raised against Human, Cynomolgus Monkey, Mouse TLR2.
- Host Species:
- Mouse
- Species Reactivity:
- Human; Cynomolgus Monkey; Mouse
- Applications:
- FC; ICC; ELISA; IP; In Vitro; Inhib; In Vivo; Antagonist
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
SPECIFIC INQUIRY
inquiryDescription
Species Reactivity
Clonality
Host Species
Applications
Relevant Diseases
Formulation
Preservatives
Concentration
Purification
Purity
Endotoxin Level
Low Endotoxin < 1 EU/mg
Shipping
Storage
Research Use Only
Target
Official Name
Full Name
Alternative Names
Figure 1 Inhibition of host activation by microbial challenge in vivo by mAb T2.5.
Mice were pretreated intraperitoneally with 1 mg mAb T2.5 (solid bars) or not (open bars). Mice were challenged with P3CSK4 and D-galactosamine (ip) 1 h later and sacrificed after 2 or 4 h (n = 4 per group per time point). Serum concentrations of TNFα (a), GROα/KC (human IL-8 homologue) (b), IL-6 (c) and IL-12p40 (d) were analyzed by ELISA (*p<0.05, **p<0.005 , ***p<0.001, Student's t-test for unconnected samples).
Figure 2 Effect of mAb T2.5 administration on viability after TLR2-specific systemic challenge.
Mice were pretreated intraperitoneally with 1 mg mAb T2.5 (solid bars) or not (open bars). Mice were challenged with P3CSK4 and D-galactosamine (ip) 1 h later and sacrificed after 2 or 4 h (n = 4 per group per time point). Serum concentrations of TNFα (a), GROα/KC (human IL-8 homologue) (b), IL-6 (c) and IL-12p40 (d) were analyzed by ELISA (*p<0.05, **p<0.005 , ***p<0.001, Student's t-test for unconnected samples).
Figure 3 Dose-dependent binding of T2.5 to mTLR2ECD and failed binding of TL2.1 to the same antigen in ELISA.
Mice were pretreated intraperitoneally with 1 mg mAb T2.5 (solid bars) or not (open bars). Mice were challenged with P3CSK4 and D-galactosamine (ip) 1 h later and sacrificed after 2 or 4 h (n = 4 per group per time point). Serum concentrations of TNFα (a), GROα/KC (human IL-8 homologue) (b), IL-6 (c) and IL-12p40 (d) were analyzed by ELISA (*p<0.05, **p<0.005 , ***p<0.001, Student's t-test for unconnected samples).
Figure 4 Lack of interaction between TL2.1 and mouse TLR2 in immunoprecipitation and subsequent western blot analysis.
Lysates of 1 × 106 mouse RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody indicated and 20 μl of protein G beads (Santa Cruz, CA, USA) were mixed for pelleting. Immune complexes were analyzed by immunoblot analysis using Flag or murine TLR2-specific antisera.
Figure 5 Inhibition of lipopeptide-induced cell activation by recombinant single-chain and partially humanized hT2.5 antibodies. HT2.5 was overexpressed in HEK293 cells and the supernatant was collected.
Lysates of 1 × 106 mouse RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody indicated and 20 μl of protein G beads (Santa Cruz, CA, USA) were mixed for pelleting. Immune complexes were analyzed by immunoblot analysis using Flag or murine TLR2-specific antisera.
Figure 6 Application of mAb T2.5 in TLR2 specific detection.
Lysates of 1 × 106 mouse RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody indicated and 20 μl of protein G beads (Santa Cruz, CA, USA) were mixed for pelleting. Immune complexes were analyzed by immunoblot analysis using Flag or murine TLR2-specific antisera.
Publications (0)
- NeuroMab™ Anti-TREM2 BBB Shuttle Antibody(NRZP-1022-ZP4114) (Cat#: NRZP-1022-ZP4114)
- NeuroMab™ Anti-Tau Antibody(NRP-0422-P1760) (Cat#: NRP-0422-P1760)
- NeuroMab™ Anti-Alpha Synuclein BBB Shuttle Antibody(NRZP-1022-ZP4050) (Cat#: NRZP-1022-ZP4050)
- NeuroMab™ Anti-SEZ6 Antibody(NRP-0422-P515) (Cat#: NRP-0422-P515)
- Mouse Anti-SCN5A Monoclonal Antibody (CBP708) (Cat#: NAB-0720-Z2720)
- NeuroMab™ Anti-ApoC3 BBB Shuttle Antibody(NRZP-1022-ZP3503) (Cat#: NRZP-1022-ZP3503)
- NeuroMab™ Anti-TREM2 Antibody(NRP-0422-P792) (Cat#: NRP-0422-P792)
- NeuroMab™ Anti-Tau Antibody(NRP-0422-P1686) (Cat#: NRP-0422-P1686)
- NeuroMab™ Mouse Anti-SHANK3 Monoclonal Antibody (CBP929) (Cat#: NAB-0720-Z3477)
- NeuroMab™ Anti-CD20 Antibody(NRP-0422-P1230) (Cat#: NRP-0422-P1230)
- Mouse Retinal Ganglion Cell Line RGC-5 (Cat#: NCL2110P154)
- iNeu™ Human Midbrain Dopaminergic Neurons (Cat#: NCL-21P6-003)
- iNeu™ Human Sensory Neurons (Cat#: NCL-2103-P62)
- Human Brain Vascular Pericytes (Cat#: NCL-21P6-015)
- iNeu™ Retinal Pigment Epithelial Cells (RPE) (Cat#: NRZP-0323-ZP92)
- Mouse Microglia Cell Line BV-2, Immortalized (Cat#: NCL2110P153)
- Mouse Hippocampal Neuron Cell HT22 (Cat#: NCL2110P001)
- iNeu™ Microglia (Cat#: NCL-7P018)
- iNeu™ Human Motor Neurons (Cat#: NCL-2103-P71)
- C57 Brain Cortex Neurons [Mouse] (Cat#: NCC20-9PZ48)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Beta Amyloid (1-40), Aggregation Kit (Cat#: NRZP-0323-ZP199)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- pAAV-syn-FLEX-jGCaMP8f-WPRE (Cat#: NTA-2106-P064)
- AAV2 Full Capsids, Reference Standards (Cat#: NTC2101070CR)
- pAAV-syn-FLEX-jGCaMP8m-WPRE (Cat#: NTA-2106-P065)
- AAV-EF1a-mCherry-flex-dtA (Cat#: NRZP-0622-ZP616)
- VSV-eGFP (Cat#: NTA-2011-ZP20)
- AAV2/9-hSyn-Flpo-EGFP-WPRE-pA (Cat#: NTA-2012-ZP149)
- pAAV-syn-FLEX-jGCaMP8s-WPRE (Cat#: NTA-2106-P066)
- Dextran-CYanine5.5 (Cat#: NTA-2011-ZP118)
- pAAV-syn-jGCaMP8s-WPRE (Cat#: NTA-2106-P063)
- PRV-CAG-EGFP (Cat#: NTA-2011-ZP14)
- Human huntingtin (HTT) (NM_002111) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0497)
- Mouse Parkinson disease (autosomal recessive, early onset) 7 (Park7) (NM_020569) clone, Untagged (Cat#: NEP-0621-R0133)
- Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2) (NM_020093) ORF clone/lentiviral particle, Myc-DDK Tagged (Cat#: NEP-0621-R0041)
- App Rat amyloid beta (A4) precursor protein (App)(NM_019288) ORF clone, Untagged (Cat#: NEP-0421-R0053)
- Human huntingtin-associated protein 1 (HAP1) transcript variant 2 (NM_177977) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0676)
- Human superoxide dismutase 1, soluble (SOD1) (NM_000454) ORF clone, TurboGFP Tagged (Cat#: NEP-0521-R0748)
- Tau Antisense Oligonucleotide (IONIS-MAPTRx) (Cat#: NV-2106-P29)
- Human superoxide dismutase 3, extracellular (SOD3) (NM_003102) ORF clone, Untagged (Cat#: NEP-0521-R0808)
- Lenti of Mouse synuclein, alpha (Snca) transcript variant (NM_001042451) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0864)
- Human apolipoprotein E (APOE) (NM_000041) ORF clone, Untagged (Cat#: NEP-0421-R0232)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein (Cat#: NRZP-0423-ZP498)
- NeuroPro™ Anti-EPO BBB Shuttle Protein (Cat#: NRZP-0423-ZP508)
- NeuroPro™ Anti-Erythropoietin BBB Shuttle Protein (Cat#: NRZP-0423-ZP499)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein (Cat#: NRZP-0423-ZP509)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein (Cat#: NRZP-0423-ZP502)
- NeuroPro™ Anti-PON1 BBB Shuttle Protein (Cat#: NRZP-0423-ZP507)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein (Cat#: NRZP-0423-ZP501)
- NeuroPro™ Anti-ASA BBB Shuttle Protein (Cat#: NRZP-0423-ZP504)
- NeuroPro™ Anti-SGSH BBB Shuttle Protein (Cat#: NRZP-0423-ZP505)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein (Cat#: NRZP-0423-ZP510)