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Creative Biolabs

NeuroMab™ Anti-CD282 Antibody(NRP-0422-P737)

[CAT#: NRP-0422-P737]

A functional antibody raised against Human, Cynomolgus Monkey, Mouse TLR2.

Host Species:
Mouse
Species Reactivity:
Human; Cynomolgus Monkey; Mouse
Applications:
ELISA; Block; Inhib; In Vitro; In Vivo

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Product Overview

Description

The antibody specifically binds to TLR2 and selectively inhibit induction of cytokines.

Species Reactivity

Human; Cynomolgus Monkey; Mouse

Clonality

Monoclonal

Host Species

Mouse

Applications

ELISA; Block; Inhib; In Vitro; In Vivo

Relevant Diseases

Alzheimer's Disease; Parkinson's Disease
Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only
Target

Target

CD282

Official Name

TLR2

Full Name

Toll-like receptor 2

Alternative Names

TLR2
Product Pictures
ELISA

Figure 1 is a bar graph of enzyme-linked immunosorbent assay (ELISA) results depicting IL-6 expression levels in human PMBC in the presence or absence of TLR1 mAb (GD2.F4) or TLR2 mAb (11G7) araLAM or PaM3CSK4 existence or non-existence.

Lysates of 1 × 106 mouse RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody indicated and 20 μl of protein G beads (Santa Cruz, CA, USA) were mixed for pelleting. Immune complexes were analyzed by immunoblot analysis using Flag or murine TLR2-specific antisera.

ELISA

Figure 2 is a graph depicting the results of experiments in which IL-8 expression levels (pg/ml) were determined in the presence of different concentrations of control antibody (OKT8), anti-CD14 (26ic), anti-TLR1 (GD2.F4), anti-TLR2 ( TL2.1), and anti-TLR1 and anti-TLR2.

Lysates of 1 × 106 mouse RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody indicated and 20 μl of protein G beads (Santa Cruz, CA, USA) were mixed for pelleting. Immune complexes were analyzed by immunoblot analysis using Flag or murine TLR2-specific antisera.

ELISA

Figure 3 is a graph depicting the presence or absence of TLR1 mAb (GD2.F4) or TLR2 mAb (11G7) and the presence or absence of zymosan or pLPS.

Lysates of 1 × 106 mouse RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody indicated and 20 μl of protein G beads (Santa Cruz, CA, USA) were mixed for pelleting. Immune complexes were analyzed by immunoblot analysis using Flag or murine TLR2-specific antisera.

ELISA

Figure 4 is a graph depicting the results of experiments in which anti-TLR1 (GD2.F4), anti-TLR2 (TL2.1) and control IgG or anti-TLR1, anti-TLR2 and anti-CD14 monoclonal antibodies. The concentration of TLR1 and TLR2 antibodies was 0.2 μg/ml. Different concentrations of isotype control or anti-CD14 antibody were used.

Lysates of 1 × 106 mouse RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody indicated and 20 μl of protein G beads (Santa Cruz, CA, USA) were mixed for pelleting. Immune complexes were analyzed by immunoblot analysis using Flag or murine TLR2-specific antisera.

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