NeuroMab™ Anti-CXCR4 BBB Shuttle Antibody(NRZP-1022-ZP3796)
- Host Species:
- Human
- Species Reactivity:
- Human
- Applications:
- FC; Inhib; In Vivo; In Vitro; Block
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Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.
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Figure 1 shows the binding of human anti-CXCR4 antibodies F7, F9, D1 and E2 to CEM cells expressing native human CXCR4 on the cell surface.
Figure 2 shows antibody competition for binding to CEM cells between FITC-labeled anti-CXCR4 antibody F9 and a panel of unlabeled human anti-CXCR4 antibodies.
Figure 3 shows flow cytometry analysis of BMS-936564 binding.
Figure 4 shows that anti-CXCR4 human antibodies F7 (BMS-936564), F9 and D1 inhibit the binding of125I-labeled CXCL12 to CXCR4 expressed on CEM cells. E2 antibody does not inhibit the binding of CXCL12 to CEM cells.
Figure 5 shows the inhibition of CXCL12(SDF-1) induced calcium flux in CEM cells by anti-CXCR4 human antibodies F7 (BMS-936564), F9 and D1. E2 did not significantly inhibit CXCL12-induced calcium flux.
Figure 6 shows inhibition of CXCL12-induced calcium flux in CXCR4+ cells by anti-CXCR4 antibody MDX-1338 (BMS-936564) or anti-CXCL12 antibody.
Calcium flux assays were performed by incubating Ramos cells with calcium 4 dye in the presence or absence of test antibodies or isotype controls. Dye-loaded cells were incubated with Ramos and CEM cells at room temperature with 50 nM and 5 nM CXCL12, respectively. The area under the fluorescence curve between 20 and 200 seconds was quantified and the EC50 was calculated.
Figure 7 shows the inhibition of CXCL12-induced migration of CEM cells by anti-CXCR4 human antibodies F7 (BMS-936564) and F9, whereas antibodies D1 and E2 did not significantly inhibit migration.
Figure 8 shows the in vitro inhibition of Ramos tumor cell proliferation by anti-CXCR4 human antibodies F7 (BMS-936564), F9 and E2.
Figure 9 shows the inhibition of Ramos tumor cell proliferation in vivo in a subcutaneous tumor model by anti-CXCR4 human antibodies F7 (BMS-936564) and F9.
Figure 10 shows the percentage survival of mice treated with anti-CXCR4 human antibody F9 or anti-CXCR4 antibody BMS-936564 in the Ramos systemic tumor cell model.
BMS-936564 was very effective in this Ramos systemic model, whereas anti-CXCL12 Ab showed no effect.
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