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Creative Biolabs

NeuroMab™ Anti-CXCR4 BBB Shuttle Antibody(NRZP-1022-ZP3799)

[CAT#: NRZP-1022-ZP3799]

Host Species:
Mouse
Species Reactivity:
Human
Applications:
FC; IHC; In Vivo; In Vitro; Inhib

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Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Applications

FC; IHC; In Vivo; In Vitro; Inhib

Relevant Diseases

Alzheimer's Disease; Parkinson's Disease
Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only
Target

Target

CXCR4

Official Name

CXCR4

Full Name

C-X-C Motif Chemokine Receptor 4

Alternative Names

NR2F1; BBOAS; BBSOAS; COUP-TFI; EAR-3; EAR3; ERBAL3; NR2F2; SVP44; TCFCOUP1; TFCOUP1; nuclear receptor subfamily 2 group F member 1; COUPTF1
Product Pictures
FuncS

Figure 1 shows the competition of specific [ 125 I]SDF1 binding to 414H5 and 515H7 Mabs on the cell membrane of CHO-K1 cells stably expressing wild-type human CXCR4 (T total binding; NS non-specific binding).

FuncS

Figure 2 shows the modulation of G protein activation by 414H5 Mab by monitoring the [35S]GTPγS binding response of wild-type CXCR4 receptors stably expressed in NIH-3T3 cells.

FuncS

Figure 3 shows the modulation of G protein activation by anti-CXCR4 monoclonal antibodies 414H5 and 515H7 by monitoring the [35S]GTPyS binding response of HeLa human tumor cells stimulated with SDF-1 (10 and 100 nM).

FuncS

Figure 4 shows that SDF-1 and 414H5 Mabs modulate the binding of CXCR4 receptor to different interaction partners by bioluminescence resonance energy transfer (BRET) method in HEK293 cells.

FuncS

Figure 5 shows the inhibition of forskolin-stimulated cAMP production by SDF-1 and 414H5 and 515H7 Mabs in NIH3T3 cells stably expressing the CXCR4 receptor.

FuncS

Figure 6 shows the modulation of G protein activation by anti-CXCR4 monoclonal antibodies 414H5 and 515H7 by monitoring the [35S]GTPγS binding response of a constitutively active mutant Asn119Ser CXCR4 receptor stably expressed in CHO-K1 cells.

FuncS

Figure 7 shows that CXCR4 Mab 414H5 inhibits SDF-1-induced migration of U937 cells in vitro.

FuncS

Figure 8 shows the inhibition of MDA-MB-231 xenograft tumor growth by anti-CXCR4 Mab 414H5 in Nod/Scid mice.

FuncS

Figure 9 shows the competition of murine m414H5 and m515H7 mAbs and chimeric mAbs c414H5 and c515H7 for specific [125I]SDF1 binding to the cell membrane of CHO-K1 cells stably expressing wild-type human CXCR4 (T total binding; NS non-specific binding).

FuncS

Figure 10 shows the modulation of G protein activation by murine m414H5 and m515H7 mAbs and chimeric mAbs c414H5 and c515H7, as monitored in NIH-3T3 cells stimulated with SDF-1 (10 nM) [35S]GTPγS binding response of stably expressed wild-type CXCR4 receptor).

FuncS

Figure 11 shows the effects of anti-CXCR4 murine m414H5 and m515H7 mAbs and chimeric mAbs c414H5 and c515H7 on G protein activation by monitoring the [35S]GTPγS binding response at HeLa human tumor cells stimulated with SDF-1 (10 nM). adjust.

FuncS

Figure 12 shows the inhibition of SDF-1-induced migration of U937 cells by CXCR4 Mabs m414H5 and c414H5 in vitro.

FuncS

Figure 13 shows the activity of anti-CXCR4 chimeric Mabs c414H5 and c515H7 in the U937 Nod/Scid mouse survival model.

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