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Creative Biolabs

NeuroMab™ Anti-TLR2 BBB Shuttle Antibody(NRZP-1022-ZP2655)

[CAT#: NRZP-1022-ZP2655]

Host Species:
Humanized
Species Reactivity:
Human; Cynomolgus Monkey; Mouse
Applications:
FC; ICC; ELISA; IP; In Vitro; Inhib; In Vivo; Antagonist

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Product Overview

Description

Brain uptake of therapeutic antibodies is severely limited by their size. To achieve enhanced BBB crossing, Creative Biolabs developed a BBB shuttle antibody platform by utilizing the endogenous macromolecule transportation pathway, known as receptor-mediated transcytosis (RMT). The engineered antibody-based carrier is believed to significantly to increase the macromolecule brain entry to combat CNS diseases.
Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.

Species Reactivity

Human; Cynomolgus Monkey; Mouse

Clonality

Monoclonal

Host Species

Humanized

Applications

FC; ICC; ELISA; IP; In Vitro; Inhib; In Vivo; Antagonist

Relevant Diseases

Alzheimer's Disease; Parkinson's Disease; Ischemic Stroke; Multiple Sclerosis; Neuroinflammation
Product Properties

Storage

Store at -20°C. Do not aliquot the antibody.

Research Use Only

For research use only
Target

Target

CD282

Official Name

TLR2

Full Name

Toll-like receptor 2

Alternative Names

TLR2
Product Pictures
FuncS

Fig.1 Inhibitory effect of mAb T2.5 on host activation by microbial challenge in vivo.

Mice were pretreated i. p. with 1 mg mAb T2.5 (solid bars) or left untreated (empty bars). Mice were challenged after 1 h with P3CSK4 and D-galactosamine (i. p.), as well as sacrificed 2 h or 4 h later (n=4 for each group at each time point). Serum concentrations of TNFα (a), GROα/KC (human IL-8 homologue) (b), IL-6 (c), and IL-12p40 (d) were analyzed by ELISA (*p<0.05, **p<0.005, ***p<0.001, student's t-test for unconnected samples).

FuncS

Fig.2 Effects of mAb T2.5 administration on viability upon TLR2-specific systemic challenging.

Mice were pretreated i. p. with 1 mg mAb T2.5 (solid bars) or left untreated (empty bars). Mice were challenged after 1 h with P3CSK4 and D-galactosamine (i. p.), as well as sacrificed 2 h or 4 h later (n=4 for each group at each time point). Serum concentrations of TNFα (a), GROα/KC (human IL-8 homologue) (b), IL-6 (c), and IL-12p40 (d) were analyzed by ELISA (*p<0.05, **p<0.005, ***p<0.001, student's t-test for unconnected samples).

ELISA

Fig.3 Dose dependent binding of T2.5 to mTLR2ECD versus failed binding of TL2.1 to the same antigen in ELISA.

Mice were pretreated i. p. with 1 mg mAb T2.5 (solid bars) or left untreated (empty bars). Mice were challenged after 1 h with P3CSK4 and D-galactosamine (i. p.), as well as sacrificed 2 h or 4 h later (n=4 for each group at each time point). Serum concentrations of TNFα (a), GROα/KC (human IL-8 homologue) (b), IL-6 (c), and IL-12p40 (d) were analyzed by ELISA (*p<0.05, **p<0.005, ***p<0.001, student's t-test for unconnected samples).

WB

Fig.4 Lack of interaction between TL2.1 and murine TLR2 in Immunoprecipitation and subsequent immunoblot analysis.

Lysates of 1×106 murine RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody as indicated, and 20 μl of protein G beads (Santa Cruz, Calif., USA) were mixed for o. n. precipitation. Immune complexes were analyzed by immunoblot analysis with either Flag- or murine TLR2-specific antiserum.

Inhib

Fig.5 Inhibitory effect of recombinant single chain and partially humanized hT2.5 antibody on lipopeptide induced cell activation. HT2.5 was overexpressed in HEK293 cells and supernatant collected.

Lysates of 1×106 murine RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody as indicated, and 20 μl of protein G beads (Santa Cruz, Calif., USA) were mixed for o. n. precipitation. Immune complexes were analyzed by immunoblot analysis with either Flag- or murine TLR2-specific antiserum.

FCM

Fig.6 Application of mAb T2.5 for specific detection of TLR2.

Lysates of 1×106 murine RAW264.7 macrophages or HEK293 cells, 1 μg of each antibody as indicated, and 20 μl of protein G beads (Santa Cruz, Calif., USA) were mixed for o. n. precipitation. Immune complexes were analyzed by immunoblot analysis with either Flag- or murine TLR2-specific antiserum.

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