NeuroMab™ Rat Anti-Mouse ADGRE1 Monoclonal Antibody (CBP1577)

Description

The rat anti-mouse F4/80 antibody recognizes the mouse F4/80 antigen, which is a member of the ~160 kDa cell surface glycoprotein of the EGF-TM7 protein family, and shares 68% of the amino acids with the mucin-like hormone receptor containing the human EGF module Identity 1 (EMR1).
The expression of F4/80 is heterogeneous and is regulated during the maturation and activation of macrophages. F4/80 antigen is expressed in a variety of mature tissue macrophages, including Kupffer cells, Langerhans cells, microglia, macrophages located in the lamina propria, peritoneal cavity, lung, thymus, and bone marrow stroma And macrophages. Spleen (Hume et al., 1984). The F4/80 antigen is also expressed on a subpopulation of dendritic cells, but not in macrophages located in the T cell area of the spleen and lymph nodes (Gordon et al., 1994). The ligand and biological functions of F4/80 antigen have not been fully determined, but some people have proposed the role of F4/80 in the generation of efferent CD8 + ve regulatory T cells (Lin et al., 2005).
The rat anti-mouse F4/80 antibody can regulate the level of cytokines produced by Listeria monocytogenes (Warschkau & Kiderlen, 1999).

Immunogen

Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2b

Clone Number

CBP1577

Applications

FC; IEM; IHC-Fr; IHC-P; IHC-Re; IP; RIA

Application Notes

FCM (1/10)

Relevant Diseases

Alzheimer's Disease

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1366

Form

Tissue Culture Supernatant - liquid

Formulation

Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
0.09% Sodium Azide

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

ADGRE1

Official Name

ADGRE1

Full Name

EGF-like module-containing mucin-like hormone receptor-like 1

Alternative Names

ADGRE1; TM7LN3; EMR1; adhesion G protein-coupled receptor E1

Gene ID

2015(Human); 13733(Mouse)

Uniprot ID

Q14246(Human); Q61549(Mouse)

1. Austyn, J.M. & Gordon, S. (1981) F4/80, a monoclonal antibody directed specifically against the mouse macrophage.
2. Hume, D.A. et al. (1984) The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80: macrophages associated with epithelia.
3. Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.
4. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues.
5. Warschkau, H. & Kiderlen, A.F. (1999) A monoclonal antibody directed against the murine macrophage surface molecule F4/80 modulates natural immune response to Listeria monocytogenes.
6. Lin, H.H.et al. (2005) The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.
7. Chan, R.J. et al. (2005) Human somatic PTPN11 mutations induce hematopoietic cell hypersensitivity to granulocyte-macrophage colony stimulating factor
8. Moore, K.J. et al. (2000) Divergent response to LPS and bacteria in CD14-deficient murine macrophages.
9. Dandekar, A.A.et al. (2004) Bystander CD8 T-cell-mediated demyelination is interferon-gamma-dependent in a coronavirus model of multiple sclerosis.
10. Muto, A. et al. (2011) Eph-B4 prevents venous adaptive remodeling in the adult arterial environment.
11. Pizza, F.X. et al. (2005) Neutrophils contribute to muscle injury and impair its resolution after lengthening contractions in mice.
12. Tarallo, V. et al. (2011) The biflavonoid amentoflavone inhibits neovascularization preventing the activity of proangiogenic vascular endothelial growth factors.
13. Rivollier, A. et al. (2012) Inflammation switches the differentiation program of Ly6Chi monocytes from antiinflammatory macrophages to inflammatory dendritic cells in the colon.
14. Hemmi, H. et al. (2009) A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells.
15. Seitz, O. et al. (2010) Wound Healing in Mice with High-Fat Diet- or ob Gene-Induced Diabetes-Obesity Syndromes: A Comparative Study
16. Miao, E.A. et al. (2011) Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria.
17. Wang, X. et al. (2011) Activation of the cholinergic antiinflammatory pathway ameliorates obesity-induced inflammation and insulin resistance
18. Cunningham, O. et al. (2009) Microglia and the urokinase plasminogen activator receptor/uPA system in innate brain inflammation.
19. Gornicka, A. et al. (2012) Adipocyte hypertrophy is associated with lysosomal permeability both in vivo and in vitro: role in adipose tissue inflammation.
20. Akbarshahi, H. et al. (2012) Enrichment of Murine CD68(+)CCR2(+) and CD68(+)CD206(+) Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.
21. Banda NK et al. (2012) Role of C3a receptors, C5a receptors, and complement protein C6 deficiency in collagen antibody-induced arthritis in mice.
22. Bonde, A.K. et al. (2012) Intratumoral macrophages contribute to epithelial-mesenchymal transition in solid tumors.
23. Choi, K.M. et al. (2010) CD206-positive M2 macrophages that express heme oxygenase-1 protect against diabetic gastroparesis in mice.
24. Tamaki, S. et al. (2013) Interleukin-16 promotes cardiac fibrosis and myocardial stiffening in heart failure with preserved ejection fraction.
25. Kihira, Y. et al. (2014) Deletion of hypoxia-inducible factor-1α in adipocytes enhances glucagon-like Peptide-1 secretion and reduces adipose tissue inflammation.
26. Chinzei, N. et al. (2015) P21 deficiency delays regeneration of skeletal muscular tissue.
27. Sumiyoshi, M. et al. (2015) Antitumor and antimetastatic actions of xanthoangelol and 4-hydroxyderricin isolated from Angelica keiskei roots through the inhibited activation and differentiation of M2 macrophages.
28. Kim, M. et al. (2015) Progression of Alport Kidney Disease in Col4a3 Knock Out Mice Is Independent of Sex or Macrophage Depletion by Clodronate Treatment.
29. Nagase, M. et al. (2016) Deletion of Rac1GTPase in the Myeloid Lineage Protects against Inflammation-Mediated Kidney Injury in Mice.
30. Bonaterra, G.A. et al. (2016) Morphological Alterations in Gastrocnemius and Soleus Muscles in Male and Female Mice in a Fibromyalgia Model.
31. Lei, B. et al. (2016) Neuroprotective pentapeptide CN-105 improves functional and histological outcomes in a murine model of intracerebral hemorrhage.
32. Glastras, S.J. et al. (2017) The renal consequences of maternal obesity in offspring are overwhelmed by postnatal high fat diet.
33. Zeng, J. & Howard, J.C. (2010) Spontaneous focal activation of invariant natural killer T (iNKT) cells in mouse liver and kidney.
34. Pepe, G. et al. (2017) Self-renewal and phenotypic conversion are the main physiological responses of macrophages to the endogenous estrogen surge.
35. Kawada, S. et al. (2017) Impairment of cold injury-induced muscle regeneration in mice receiving a combination of bone fracture and alendronate treatment.
36. Zhang, M.Z. et al. (2015) Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension.
37. Crompton, M. et al. (2017) A mutation in Nischarin causes otitis media via LIMK1 and NF-κB pathways.
38. Sogawa, Y. et al. (2017) Infiltration of M1, but not M2, macrophages is impaired after unilateral ureter obstruction in Nrf2-deficient mice.
39. Suzuki, Y. et al. (2017) Requisite role of vasohibin-2 in spontaneous gastric cancer formation and accumulation of cancer-associated fibroblasts.
40. Peng, Y. (2018) B cell responses to apoptotic cells in MFG-E8-/- mice.
41. WasgewatteWijesinghe, D.K. et al. (2019) Normal inflammation and regeneration of muscle following injury require osteopontin from both muscle and non-muscle cells.
42. Wang, H. et al. (2019) Embelin can protect mice from thioacetamide-induced acute liver injury.
43. Maydan, O. et al. (2018) Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy.