NeuroMab™ Hamster Anti-Notch2 Monoclonal Antibody (CBP1582)

Description

The hamster anti-Mouse Notch 2 antibody recognizes one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, and the Notch 2 is activated by binding to a membrane-bound specific ligand containing the DSL domain. Notch signaling pathway is an evolutionary conserved pathway in multicellular organisms. It is essential for cell-to-cell communication in basic developmental and physiological processes (including regulation of neurons, heart and endocrine development, stem cell hematopoiesis, thymic T cell development, and Tumor progression and suppression. The Notch receptor is connected by its specific ligands Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, and is physically adjacent to the signal-receiving cell through the ADAM family of metalloproteinases and γ-receptors Induces proteolysis of the receptor. The secretin complex in the transmembrane domain releases the Notch intracellular domain (NICD), which translocates to the nucleus. Then, subsequent signal transduction occurs through the CSL-NICD-Mastermind complex cascade (standard pathway) or the NF-kappaB-NICD and CSL-NICD-Deltex complex signal cascade (non-classical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, while the non-canonical pathway promotes differentiation.

Immunogen

Mouse Notch 2-Fc fusion protein.

Species Reactivity

Mouse; Rat

Clonality

Monoclonal

Host Species

Hamster

Isotype

IgG

Clone Number

CBP1582

Applications

FC

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1370

Form

Liquid

Formulation

PBS
0.09% Sodium Azide

Concentration

1 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein G.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

NOTCH2

Official Name

Notch2

Full Name

Notch 2

Alternative Names

Notch 2; HN2; Neurogenic Locus Notch Homolog Protein 2; Notch (Drosophila) Homolog 2; Notch Homolog 2 (Drosophila); Notch Homolog 2; HJCYS; AGS2

Gene ID

4853(Human); 18129(Mouse)

Uniprot ID

Q04721(Human); O35516(Mouse)

1. Moriyama, Y. et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice. 2. Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands. 3. Gibb, D.R. et al. (2010) ADAM10 is essential for Notch2-dependent marginal zone B cell development and CD23 cleavage in vivo. 4. Sakata-Yanagimoto, M. et al. (2011) Notch2 signaling is required for proper mast cell distribution and mucosal immunity in the intestine. 5. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex. 6. Iso, T. et al. (2003) Notch signaling in vascular development. 7. Hu, X. et al. (2008) Integrated regulation of Toll-like receptor responses by Notch and interferon-gamma pathways. 8. Hoyne, G.F. et al. (2001) Notch signalling in the regulation of peripheral immunity.