NeuroMab™ Mouse Anti-Human STAT3 Monoclonal Antibody (CBP2256)

Product Overview

Description

The STAT3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors and is required for murine fetal development. STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to a cytokine receptor-associated kinase activity. Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding. Transcriptional activation was reported to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways.

Immunogen

Human Stat3 peptide phosphorylated at Ser 727. Complete Freund's adjuvant.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2256

Applications

Application Notes

FCM (5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1670

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA.

Concentration

Batch Dependent

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

The antibody solution should be stored undiluted between 2°C and 8°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

STAT3 Phospho (Ser727)

Official Name

STAT3

Full Name

Signal transducer and activator of transcription 3

Alternative Names

Signal transducer and activator of transcription 3; Acute-phase response factor (APRF); HIES; ADMIO

Gene ID

6774(Human); 20848(Mouse); 25125(Rat)

Uniprot ID

P40763(Human); P42227(Mouse); P52631(Rat)

References

1. Akira S, et al. 1994. Cell. 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T, et al. 1999. Adv. Immunol. 71:145.

Publications