Mouse Anti-STAT3 Monoclonal Antibody (CBP2638)

Product Overview

Description

Signal transducer and activator of transcription 3 (acute-phase response factor)is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3.

Expression Host

Mammalian cells

Immunogen

STAT3 fusion protein Ag0360

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

CBP2638

Applications

IF; IHC; IP; WB; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200; IP : 0.5-4.0 ug; WB : 1:500-1:1000

Research Areas

Neural Signal Transduction

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1093 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

STAT3 Phospho (Ser727)

Official Name

STAT3

Full Name

Signal transducer and activator of transcription 3

Alternative Names

Acute phase response factor; APRF; HIES; STAT3

Gene ID

6774(Human); 20848(Mouse); 25125(Rat)

Uniprot ID

P40763(Human); P42227(Mouse); P52631(Rat)

References

1. Chen, B., Yang, L., Chen, J., Chen, Y., Zhang, L., Wang, L.,... & Yu, H. (2019). Inhibition of Connexin43 hemichannels with Gap19 protects cerebral ischemia/reperfusion injury via the JAK2/STAT3 pathway in mice. Brain Research Bulletin, 146, 124-135. 2. Cheng, Z., Lei, Z., Yang, P., Si, A., Xiang, D., Tang, X.,... & Hüser, N. (2019). Exosome-transmitted p120-catenin suppresses hepatocellular carcinoma progression via STAT3 pathways. Molecular carcinogenesis, 58(8), 1389-1399. 3. Huang, R., Wang, S., Wang, N., Zheng, Y., Zhou, J., Yang, B.,... & Chen, Z. (2020). CCL5 derived from tumor-associated macrophages promotes prostate cancer stem cells and metastasis via activating β-catenin/STAT3 signaling. Cell Death & Disease, 11(4), 1-20.

Publications