NeuroMab™ Mouse Anti-STAT3 Monoclonal Antibody (CBP2398)

Product Overview

Description

STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to a cytokine receptor-associated kinase activity. Phosphorylation of STAT3 induces nuclear translocation to activate transcription. STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.

Immunogen

Purified recombinant fragment (aa 20-240) of human STAT3 expressed in E. coli.

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2398

Applications

ChIP; WB

Application Notes

ChIP (1:50-1:150); WB (0.5-1.0 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1764

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.05% sodium azide

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

STAT3 Phospho (Ser727)

Official Name

STAT3

Full Name

Signal transducer and activator of transcription 3

Alternative Names

STAT3; ADMIO; APRF; HIES; signal transducer and activator of transcription 3; ADMIO1

Gene ID

6774(Human); 20848(Mouse); 25125(Rat)

Uniprot ID

P40763(Human); P42227(Mouse); P52631(Rat)

References

1. Akira S, et al. 1994. Cell. 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V & Martin-Romero C. 2001. Cell Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T & Grusby MJ. 1999. Adv. Immunol. 71:145.

Publications