NeuroMab™ Mouse Anti-Human STAT3 Monoclonal Antibody (CBP2254)

Product Overview

Description

Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.

Immunogen

Human STAT3 peptide phosphorylated at Y705. Complete Freund's adjuvant.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2254

Applications

ChIP; WB; FC

Application Notes

ChIP (1:50); WB (0.05-0.5 µg/mL); FCM (≤ 0.5 µg per million cells in 100 µl volume)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1669

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.05% sodium azide

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

STAT3 Phospho (Ser727)

Official Name

STAT3

Full Name

Signal transducer and activator of transcription 3

Alternative Names

Signal transducer and activator of transcription 3; Acute-phase response factor; APRF; HIES

Gene ID

6774(Human); 20848(Mouse); 25125(Rat)

Uniprot ID

P40763(Human); P42227(Mouse); P52631(Rat)

References

1. Akira S, et al. 1994. Cell. 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T, et al. 1999. Adv. Immunol. 71:145.

Publications