NeuroMab™ Rat Anti-Human ATF6 Monoclonal Antibody (CBP2468)

Product Overview

Description

ATF6 encodes a transcription factor that is anchored in the endoplasmic reticulum (ER) and activated during the unfolded protein response (UPR) to protect cells from ER stress. Under conditions of ER stress, ATF6 is transported from the ER to the Golgi apparatus, where it is cleaved by Golgi-resident proteases to release the cytosolic DNA-binding portion. Then the processed ATF6 moves to the nucleus to activate gene expression. Deletion of the isoform activating transcription factor 6α (ATF6α) and its paralog ATF6β results in embryonic lethality and notochord dysgenesis in nonhuman vertebrates, and loss-of-function mutations in ATF6α are associated with malformed neuroretina and congenital vision loss in humans. Altered ATF6 function in neurodegenerative disorders such as Parkinson's disease has been observed.

Immunogen

Human ATF6α recombinant protein (1-250 a.a.) expressed in E. coli.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2a

Clone Number

CBP2468

Applications

WB; ICC

Application Notes

WB (0.5- 5.0 µg/mL); ICC (5.0- 10.0 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1806

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

Batch Dependent

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

ATF6

Official Name

ATF6

Full Name

Activating transcription factor 6

Alternative Names

Cyclic AMP-dependent transcription factor ATF-6 alpha; ATF-6 alpha; Activating transcription factor 6 alpha; ATF6-alpha

Gene ID

22926 (Human); 226641 (Mouse)

Uniprot ID

P18850 (Human); F6VAN0 (Mouse)

References

1. Shen J, et al. 2002. Dev Cell. 3:99-111
2. Ron D and Walter P. 2007. Nat Rev Mol Cell Biol. 8: 519-529
3. Credle JJ, et al. 2015. Neurobiol Dis. 76: 112-125
4. Jin JK, et al. 2017. Circ Res. 120: 862-875
5. Kroeger H, et al. 2018. Sci Signal. 11: 5785

Publications