NeuroMab™ Mouse Anti-CD14 Monoclonal Antibody (CBP2526)

Description

Mouse anti-pig CD14, recognizes porcine CD14. At the third international symposium on porcine leukocyte differentiation antigens, clone MIL2 was clustered as porcine CD14 (Haverson et al., 2001). The mouse anti-pig CD14 clone MIL2 immunoprecipitated a protein of about 50 kDa, which was consistent with the expected apparent molecular weight of porcine CD14, and the expected CD14 spectrum was proved by double labeling and competition studies. In addition, pre-incubation of peripheral blood mononuclear cells with MIL2 inhibited the binding of FITC-labeled LPS, which is consistent with masking the CD14 LPS binding site (Thacker et al., 2001). The mouse anti-pig CD14 clone MIL2 was shown by flow cytometry to show the staining of monocytes and neutrophils in peripheral blood. Its expression pattern is similar to that of the anti-human CD14 clone TüK4. Lymphocytes and eosinophils stained MIL2. Negative (Zelnickova et al. 2007). Compared with other mammalian CD14, the cloning and identification of porcine CD14 showed a high degree of functional and structural conservation. The gene is located on chromosome 2 and is expressed in a wide range of tissues in a manner consistent with expression on myeloid cells. (Petersen et al., 2007; Sanz et al., 2007).

Immunogen

Pig peripheral blood lymphocytes.

Species Reactivity

Human; Porcine

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b

Clone Number

CBP2526

Applications

FC

Relevant Diseases

Alzheimer's Disease; Neurodegenerative Disease

Research Areas

Neuroregeneration

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1848

Form

Liquid

Formulation

PBS, 0.09% Sodium Azide, 1% BSA

Preservatives

YES

Concentration

0.1 mg/ml

Purification

Protein G

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD14

Official Name

CD14

Full Name

CD14 Molecule

Alternative Names

CD14 Molecule; CD14 Antigen; Myeloid Cell-Specific Leucine-Rich Glycoprotein

Gene ID

929(Human); 12475(Mouse); 60350(Rat)

Uniprot ID

P08571(Human); P10810(Mouse); Q63691(Rat)

1. Hauet, T. et al. (2000) Trimetazidine reduces renal dysfunction by limiting the cold ischemia/reperfusion injury in autotransplanted pig kidneys.
2. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
3. Thorgersen, E.B. et al. (2010) CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
4. Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys.
5. Li, Y. et al. (2014) Identification of apoptotic cells in the thymus of piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus.