NeuroMab™ Mouse Anti-CD14 Monoclonal Antibody (CBP2523)

Product Overview

Description

Mouse anti-human CD14 antibody recognizes a cell surface antigen of about 55 kDa, called CD14. The CD14 molecule is mainly found on monocytes and macrophages in flow cytometry, and is weakly expressed on granulocytes, but on stem cells But there is no very early differentiation state in myeloid cells. In immunohistology, CD14 molecules were found in Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. It is known that antibodies against CD14 molecules can induce the formation of oxidative bursts. In the tonsil tissue section, UCHM1 and monocytes, macrophages and dendritic reticular cells in the intervesicular tissue seen under the capsule produced positive staining reactions. Skin Langerhans cells are always negative (Hogg et al. 1984). UCHM1 also reacts with Kupffer cells and sinus wall cells in the liver.

Immunogen

Human thymocytes, followed by peripheral blood mononuclear cells.

Species Reactivity

Human; Cynomolgus monkey; Rhesus Monkey; Fish; Trout

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

CBP2523

Applications

Relevant Diseases

Alzheimer's Disease; Neurodegenerative Disease

Research Areas

Neuroregeneration

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1847

Product Properties

Form

Lyophilized

Formulation

PBS, 0.09% Sodium Azide, 1% BSA, 5% Sucrose

Preservatives

YES

Purification

Protein G

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Prior to reconstitution store at +4°C. After reconstitution store at +4°C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.

Handling Advice

Pack Size: 25 Tests/0.25ml
Reconstitute with 0.25 ml distilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD14

Official Name

CD14

Full Name

CD14 Molecule

Alternative Names

CD14 Molecule; CD14 Antigen; Myeloid Cell-Specific Leucine-Rich Glycoprotein

Gene ID

929(Human); 12475(Mouse); 60350(Rat)

Uniprot ID

P08571(Human); P10810(Mouse); Q63691(Rat)

References

1. Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
2. Köller, M. et al. (2004) Phenotypic and functional deficiencies of monocyte-derived dendritic cells in systemic lupus erythematosus (SLE) patients.
3. Kämmerer, U. et al. (2003) Unique appearance of proliferating antigen-presenting cells expressing DC-SIGN (CD209) in the decidua of early human pregnancy.
4. Goddard, S. et al. (2004) Interleukin-10 secretion differentiates dendritic cells from human liver and skin.
5. Bournazos, S. et al. (2008) Monocyte functional responsiveness after PSGL-1-mediated platelet adhesion is dependent on platelet activation status.

Publications