pAAV-FLEX-EGFPL10a

Excellent

Perfect for targeted expression in Cre lines

The pAAV-FLEX-EGFP-L10a vector worked exactly as expected in our transgenic Cre mice. The expression was highly specific, and the fluorescence was strong. It made isolating ribosome-bound mRNA for TRAP-seq straightforward. The packaging process was also smooth, and we obtained high-titer AAVs with minimal troubleshooting.

Excellent

Great fluorescent signal for cell sorting

We used this vector to express EGFP-tagged ribosomes in a targeted population of neurons. The resulting fluorescent signal was strong and clear, which made cell sorting much easier. The L10a tag performed reliably during ribosome immunoprecipitation, giving us consistent and reproducible data. A fantastic tool for TRAP experiments!

Excellent

Highly efficient in Cre-dependent systems

We tested this vector in both in vivo and in vitro systems and found that it produced reliable, high-level expression in Cre-expressing cells. The flexibility and efficiency of the AAV system allowed us to easily adapt it to our experimental designs. The EGFP-L10a fusion performed well, offering clear fluorescent labeling and effective ribosome immunoprecipitation.

Excellent

Robust and consistent expression in neurons

The expression was robust in neurons, and the fusion protein allowed us to purify ribosome-bound mRNAs with high specificity.

Excellent

Reliable and high-titer packaging

We used this vector for targeted gene expression in a Cre-dependent manner and were pleased with the results. The packaging was straightforward, and we obtained high-titer AAVs without any complications. The EGFP fluorescence was bright, and the L10a tag performed as expected during ribosome pulldowns. A very reliable tool.