pAAV-FLEX-EGFPL10a

Product Overview

Description

Cre-dependent EGFPL10a (EGFP-labeled ribosomal protein) expression can be used for translational ribosomal affinity purification (TRAP). The purity of these AAV preparations is suitable for injection into animals.

Tracer Type

Fluorescent labeling

Serotype

AAV9

Virus Type

Adeno-Associated Virus (AAV)

Applications

Control

Sub Type

AAV5; AAV Retro

Research Areas

Neural Circuit Mapping

Promoter

Ef1α

Key Components

pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

Expression

Cre-dependent

Properites

Titer

≥ 7×10¹² vg/mL

Fluorophore

EGFP

Shipping

Viral particles are shipped frozen on dry ice.

Handling Advice

The lentivirus is generally referred to as BSL-2. AAV is usually considered BSL-1, but may require BSL-244 handling depending on the insert.

Storage

Store at -80°C. Thaw just before use and keep on ice.

Quality Control

Creative Biolabs ensures high-quality viral vectors by optimizing and standardizing production plans and implementing strict quality control (QC). The specific QC analysis performed for each virus batch is different.

Titer: The precise titer of the sample will be reported on the test tube. The potency you see on this page is the guaranteed minimum potency.

Research Use Only

For research use only.

Gene/Insert

Gene/Insert Name

EGFPL10a

Species

Mouse

Insert Size (bp)

1407

Tag / Fusion Protein

GFP (N terminal on insert)

Growth in Bacteria

Bacterial Resistance(s)

Ampicillin

Growth Temperature

37°C

Growth Strain(s)

NEB Stable

Copy number

High Copy

Cloning Information

Cloning method

Restriction Enzyme

5′ cloning site

Asci (not destroyed)

3′ cloning site

NheI (not destroyed)

5′ sequencing primer

EF1a-Fwd

3′ sequencing primer

WPRE-Rev

References

Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi: 10.1016/j.celrep.2017.03.048.

Product Pictures

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Perfect for targeted expression in Cre lines

The pAAV-FLEX-EGFP-L10a vector worked exactly as expected in our transgenic Cre mice. The expression was highly specific, and the fluorescence was strong. It made isolating ribosome-bound mRNA for TRAP-seq straightforward. The packaging process was also smooth, and we obtained high-titer AAVs with minimal troubleshooting.

Excellent

Great fluorescent signal for cell sorting

We used this vector to express EGFP-tagged ribosomes in a targeted population of neurons. The resulting fluorescent signal was strong and clear, which made cell sorting much easier. The L10a tag performed reliably during ribosome immunoprecipitation, giving us consistent and reproducible data. A fantastic tool for TRAP experiments!

Excellent

Highly efficient in Cre-dependent systems

We tested this vector in both in vivo and in vitro systems and found that it produced reliable, high-level expression in Cre-expressing cells. The flexibility and efficiency of the AAV system allowed us to easily adapt it to our experimental designs. The EGFP-L10a fusion performed well, offering clear fluorescent labeling and effective ribosome immunoprecipitation.

Excellent

Robust and consistent expression in neurons

The expression was robust in neurons, and the fusion protein allowed us to purify ribosome-bound mRNAs with high specificity.

Excellent

Reliable and high-titer packaging

We used this vector for targeted gene expression in a Cre-dependent manner and were pleased with the results. The packaging was straightforward, and we obtained high-titer AAVs without any complications. The EGFP fluorescence was bright, and the L10a tag performed as expected during ribosome pulldowns. A very reliable tool.

Q&As

Can I use pAAV-FLEX-EGFPL10a for in vivo experiments?

Yes, pAAV-FLEX-EGFPL10a is suitable for in vivo studies. Its AAV vector enables efficient transduction of neurons, and the FLEX design ensures Cre-dependent expression. Researchers often use it in animal models to study gene expression in defined neuronal populations under physiological or pathological conditions, making it highly applicable for in vivo neuroscience research.

What is the typical timeline for expression after transduction with pAAV-FLEX-EGFPL10a?

After transduction with pAAV-FLEX-EGFPL10a, the expression of the EGFP-tagged L10a protein generally begins within 1-2 weeks, depending on the viral titer and the efficiency of Cre recombinase activity. Full expression is typically observed within 3-4 weeks, allowing sufficient time for subsequent RNA isolation and transcriptomic analysis in targeted neuronal populations.

What are the key considerations when planning an experiment with pAAV-FLEX-EGFPL10a?

When planning experiments with pAAV-FLEX-EGFPL10a, key factors include selecting the appropriate Cre-driver line for cell-type specificity, optimizing viral titer for efficient transduction, and timing the RNA isolation to capture dynamic changes in gene expression. Additionally, validating the expression and functionality of the EGFP-L10a construct is essential before proceeding with large-scale transcriptomic studies. Proper controls, such as using non-Cre-expressing animals, are also critical to ensure specificity and avoid background expression.