Product

beta2 adrenoceptor Assay Cell Line

[CAT#: NCL2110P306]

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Product Overview

Description

Cell line information: ADRβ2 cAMP Maxensor cell line
Official Full Name: Adrenoreceptor beta 2
DNA Accession Number:
Host Cell: U2OS
Resistance: G418 + Puromycin
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen

Features

The cAMP Maxensor ADRβ2 contains U2OS cells stably expressing cAMP Maxensor-FP650 biosensor and adrenergic receptor beta 2 (unlabeled). The Creative Biolabs cAMP Maxensor ADRβ2 cell line is designed to determine compounds or analyze their ability to modulate adrenergic receptor β2. When the agonist binds to ADRβ2, the G protein is activated, which in turn triggers a cellular response mediated by cAMP. This cell line has been verified by analyzing the intracellular distribution of cAMP Maxensor biosensor by measuring the increase of cAMP in the cytosol. This cell line allows image analysis of compound-induced stimuli. The use of isoproterenol as an agonist validates this highly reproducible assay in high-content analysis (HCA) and high-throughput analysis (HTA).

Application Notes

About Red cAMP Maxensor Biosensor:
The red cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before stimulating cAMP production, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the red cAMP Maxensor biosensor, which promotes the relocation of cells in the transport of cell vesicles. In cell lines that co-express the red cAMP Maxensor biosensor and the GPCR of interest, the activity of living cells can be easily quantified by fluorescence granularity image analysis or fluorescence intensity analysis.

Research Areas

GPCR

Assay

beta2 adrenoceptor Assay

Assay Description

β-2 adrenergic receptor (β2 adrenergic receptor); also known as ADRB2, is a β-adrenergic receptor that spans cell membranes. The beta2 adrenergic receptor test (ADRB2) from Creative Biolabs allows the use of Maxensor biosensor technology to measure ADRB2 receptor activation. The beta2 adrenergic receptor signal is stimulated by trimerized Gs protein, adenylate cyclase that increases the interaction of cAMP and downstream L-type calcium channels. The cAMP-Maxensor biosensor measures receptor activation through cAMP accumulation.

Assay Characteristics

Readout: cAMP Flux

Agonist: Isoproterenol

EC50 Agonist: 4.91 x 10⁶-10 M for cAMP Flux

Type of Assay: Cell-based / Functional

Detection Method: Fluorimetry

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.