Product

Human ATP10A Knockout A549 Cell Lysate

[CAT#: NCL2008ZP410]

Human ATP10A knockout cell lysate

Species:: Human

Cell Types:: A549 Cell

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Product Overview

Description

This is a A549 cell transfected lysate in which Human ATP10A has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

A549 Cell

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 4 bp deletion in exon2.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Growth and Development

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X,Y
D5S818: 11
D13S317: 11
D7S820: 8, 11
D16S539: 11, 12
vWA: 14
TH01: 8,9.3
TPOX: 8,11
CSF1PO: 10, 12

Tissue Source

Lung

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

ATP10A

Official Name

ATP10A

Full Name

ATPase Phospholipid Transporting 10A (Putative)

Alternative Names

ATPase Phospholipid Transporting 10A (Putative); ATP10C; P4-ATPase Flippase Complex Alpha Subunit ATP10A; Aminophospholipid Translocase VA; EC 3.6.3.1; ATPVA; ATPVC; ATPase Type IV; Phospholipid Transporting (P-Type);

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