Human CHO-K1/Gα15 Stable Cell Line

[CAT#: NCL20120141CR]

Gα15 Stable Cell Line

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Request Pricing

Inquiry

Product Overview

Description

It is a CHO-K1 cell line stably expressing the Gα15 alpha subunit protein which a Gq protein. It is used as a host cell for transfection expression of Gs and Gi/o -coupled receptors, the constitutively expressed Gα15 protein in the cells allows many transfected receptors which normally stimulate/inhibit the cAMP pathway, to couple to Gq signal transduction and mobilize intracellular calcium. The cell line carries the hygromycin B resistance gene and is resistant to hygromycin B.

Cell Types

Other Cells

Applications

GPCR Screening

Research Areas

GPCR

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen cells

Culture Medium

Ham's F12, 10% FBS, 100 μg/ml Hygromycin B

Freeze Medium

45% culture medium, 45% FBS, 10% DMSO

Family

Control

Strain

Gα15 Cell Line

Cell Purity

>95%

Shipping

Dry ice

Storage

Liquid nitrogen immediately upon delivery

Handling Advice

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Target Details

Target

GNA15

Official Name

GNA15

Alternative Names

Gα15; Gα15 alpha subunit protein

Gene ID

10181(Human); 83486(Mouse); 300996(Rat)

Uniprot ID

P52756(Human); Q91YE7(Mouse); B2GV05(Rat)

References

1. Mi, G., Liu, S., Zhang, J., Liang, H., Gao, Y., Li, N.,.. & Yang, Z. (2017). Levo-tetrahydroberberrubine produces anxiolytic-like effects in mice through the 5-HT 1A receptor. PloS one, 12(1), e0168964.

Publications