Human CHO-K1/GLP1R/Gα15 Stable Cell Line

[CAT#: NCL20120065CR] Review(5) Q&As(3)

Glucagon-like Peptide 1 Receptor Stable Cell Line

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

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Product Overview

Description

GLP1R binds specifically the glucagon-like peptide-1 (GLP1) and has much lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon. GLP1R is known to be expressed in pancreatic beta cells. Activated GLP1R stimulates the adenylyl cyclase pathway which results in increased insulin synthesis and release of insulin. Consequently, GLP1R has been suggested as a potential target for the treatment of diabetes. GLP1R is also expressed in the brain where it is involved in the control of appetite. Furthermore, mice which over express GLP1R display improved memory and learning.

Cell Types

Other Cells

Applications

GPCR Screening

Research Areas

GPCR

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen cells

Culture Medium

Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B

Freeze Medium

45% culture medium, 45% FBS, 10% DMSO

Receptor

GLP1R

Family

Glucagon receptor

Strain

GLP1R/Gα15 Cell Line

Cell Purity

>95%

Shipping

Dry ice

Storage

Liquid nitrogen immediately upon delivery

Handling Advice

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Target Details

Target

GLP1R

Official Name

GLP1R

Alternative Names

GLP-1 receptor; Glip; RATGL1RCP; GLP-1-R; GLP-1R; glucagon-like peptide 1 receptor; pancreatic beta cell receptor for the gluco-incretin hormone glucagon-like peptide 1; GLP1Rc

Gene ID

14652(Mouse); 25051(Rat)

Uniprot ID

O35659(Mouse); P32301(Rat)

References

1. Kim, M., Tan, Y. S., Cheng, W. C., Kingsbury, T. J., Heimfeld, S., & Civin, C. I. (2015). MIR 144 and MIR 451 regulate human erythropoiesis via RAB 14. British journal of haematology, 168(4), 583-597. 2. Hildebrand, M. S., Phillips, A. M., Mullen, S. A., Adlard, P. A., Hardies, K., Damiano, J. A.,.. & Hendrickx, R. (2015). Loss of synaptic Zn 2+ transporter function increases risk of febrile seizures. Scientific reports, 5, 17816.

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

This stable cell line was crucial for our drug discovery project targeting GPCR pathways

We used the Human CHO-K1/GLP1R/Gα15 Stable Cell Line for GPCR screening assays, and it performed exceptionally well. The cells showed robust expression of GLP1R and responded consistently to ligand stimulation, making our experiments reliable and reproducible.

Excellent

A valuable tool for advancing research

The cells maintained stable characteristics over multiple passages, and the expression levels of Gα15 were ideal for our assays. The cells exhibited high viability and expression of the target receptors, facilitating accurate and insightful data collection. Highly recommended for anyone studying GPCR signaling.

Excellent

Using these cells simplified our experimental setup significantly

They responded predictably to GLP1R agonists and antagonists, which was crucial for our functional assays. It provided a solid foundation for our research, demonstrating consistent responsiveness and robust expression levels. A worthwhile investment for any lab conducting GPCR research.

Excellent

Definitely recommended for similar studies

We chose this cell line for its well-characterized expression profile and suitability for our screening assays. The results were consistent across multiple experiments, validating its reliability and suitability for our drug discovery efforts.

Excellent

It offered reliable performance and consistent results

Our lab relies on accurate and reproducible data, and this cell line delivered on both fronts. It provided a reliable platform for studying GPCR interactions, demonstrating robust responses to ligand treatments. The cell line’s stability and responsiveness were instrumental in identifying lead compounds for further development.

Q&As

What type of assay formats can be used with this cell line for GPCR screening?

This cell line supports various assay formats, including calcium flux assays, cAMP accumulation assays, and reporter gene assays, which are common in GPCR research. Calcium flux assays, facilitated by the Gα15 coupling, are particularly popular due to their sensitivity and ability to detect receptor activation rapidly. The choice of assay format depends on your specific research objectives and the pharmacological properties of the ligands being tested.

Are there any recommended controls or companion cell lines for experiments?

It is recommended to use a wild-type CHO-K1 cell line as a negative control in experiments involving the CHO-K1/GLP1R/Gα15 cell line. This helps differentiate specific GLP-1R-mediated responses from background cellular activity. Additionally, using a known GLP-1R agonist as a positive control can validate the assay setup and confirm receptor functionality. Such controls are crucial for ensuring the accuracy and reliability of your experimental results.

How does this cell line compare with others for GPCR screening purposes?

The CHO-K1/GLP1R/Gα15 cell line is specifically engineered to express GLP-1 receptors, making it highly suitable for targeted GPCR studies compared to other general-purpose cell lines. Its stable expression of Gα15 enhances calcium signaling, providing a robust response to ligand stimulation. This makes it particularly advantageous for high-throughput screening applications, offering sensitivity and specificity that may not be achievable with other cell lines lacking these modifications.