Human CHO-K1/KiSS1 Stable Cell Line

[CAT#: NCL20120077CR]

KiSS1-derived Peptide Receptor Stable Cell Line

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

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Product Overview

Description

The KiSS1-derived peptide receptor (also known as GPR54 or the Kisspeptin receptor) is a Gq-coupled receptor which binds the peptide hormone kisspeptin (metastin). The KiSS1 gene inhibits metastatic activity of melanoma and other tumor cell lines, and clinical evidence supports a role for KiSS1 in inhibition of metastasis in human cancer. Kisspeptins and GPR54 also play a central role in hypothalamic regulation of puberty, by directly governing the release of gonadotropin-releasing hormone from the hypothalamus. In addition, mutations in GPR54 in mice and humans result in hypogonadotropic hypogonadism.

Cell Types

Other Cells

Applications

GPCR Screening

Research Areas

GPCR

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen cells

Culture Medium

Ham's F12, 10% FBS, 400 μg/ml G418

Freeze Medium

45% culture medium, 45% FBS, 10% DMSO

Receptor

KiSS1/GPR54

Family

Kisspeptin receptor

Strain

KiSS1 Cell Line

Cell Purity

>95%

Shipping

Dry ice

Storage

Liquid nitrogen immediately upon delivery

Handling Advice

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Target Details

Target

Kisspeptin

Official Name

KiSS1

Alternative Names

KISS1R; GPR54; Metastin receptor; AXOR12; OT7T175

Gene ID

3814(Human); 280287(Mouse); 289023(Rat)

Uniprot ID

Q15726(Human); Q6Y4S4(Mouse); Q7TSB7(Rat)

References

1. Montagna, E., Crux, S., Luckner, M., Herber, J., Colombo, A. V., Marinković, P.,.. & Sgobio, C. (2019). In vivo Ca2+ imaging of astrocytic microdomains reveals a critical role of the amyloid precursor protein for mitochondria. Glia, 67(5), 985-998. 2. Xu, Y., Wu, X., Wang, S., Yang, C., Li, Y., & Cao, Y. (2019). Hydroxyapatite Nanoparticle-Crosslinked Peptide Hydrogels for Three-Dimensional Culture and Differentiation of MC3T3-E1 Osteoblasts. Journal of biomedical nanotechnology, 15(12), 2351-2362. 3. Lan, J., Rollins, J. A., Zang, X., Wu, D., Zou, L., Wang, Z.,.. & Chen, D. (2019). Translational regulation of non-autonomous mitochondrial stress response promotes longevity. Cell reports, 28(4), 1050-1062.

Publications