Human CHO-K1/M2/Gα15 Stable Cell Line

[CAT#: NCL20120008CR] Review(5) Q&As(3)

Muscarinic Acetylcholine Receptor M2 Stable Cell Line

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

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Product Overview

Description

Muscarinic acetylcholine receptors can interact with G-proteins to initiate intracellular responses. Five muscarinic receptor subtypes have been identified and named from M1 to M5. The M2 muscarinic receptor couples to Gi/o to inhibit cAMP production.

Cell Types

Other Cells

Applications

GPCR Screening

Research Areas

GPCR; Neural Stem Cell

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen cells

Culture Medium

Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B

Freeze Medium

45% culture medium, 45% FBS, 10% DMSO

Receptor

M2/CHRM2

Family

Acetylcholine receptor (muscarinic)

Strain

M2/Gα15 Cell Line

Cell Purity

>95%

Shipping

Dry ice

Storage

Liquid nitrogen immediately upon delivery

Handling Advice

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Target Details

Target

CHRM2

Official Name

CHRM2

Alternative Names

M2 receptor; m2; acetylcholine receptor; muscarinic 2; Acm2; 7TM receptor; M2 muscarinic acetylcholine receptor; cholinergic receptor; muscarinic 2; cholinergic receptor; muscarinic 2; cardiac; muscarinic acetylcholine receptor M2; muscarinic receptor m2; AChR M2; muscarinic acetylcholine receptor 2; Chrm-2

Gene ID

1129(Human); 243764(Mouse); 81645(Rat)

Uniprot ID

P08172(Human); Q9ERZ4(Mouse); P10980(Rat)

References

1. Montagna, E., Crux, S., Luckner, M., Herber, J., Colombo, A. V., Marinković, P.,.. & Sgobio, C. (2019). In vivo Ca2+ imaging of astrocytic microdomains reveals a critical role of the amyloid precursor protein for mitochondria. Glia, 67(5), 985-998. 2. Xu, Y., Wu, X., Wang, S., Yang, C., Li, Y., & Cao, Y. (2019). Hydroxyapatite Nanoparticle-Crosslinked Peptide Hydrogels for Three-Dimensional Culture and Differentiation of MC3T3-E1 Osteoblasts. Journal of biomedical nanotechnology, 15(12), 2351-2362. 3. Lan, J., Rollins, J. A., Zang, X., Wu, D., Zou, L., Wang, Z.,.. & Chen, D. (2019). Translational regulation of non-autonomous mitochondrial stress response promotes longevity. Cell reports, 28(4), 1050-1062.

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

We plan to continue using this cell line for future studies

We have been using the human CHO-K1/M2/Gα15 cell line for several months now, and it has proven to be an invaluable tool for our GPCR screening assays. The cell line exhibits robust expression and functional responses, which are crucial for our high-throughput screening processes.

Excellent

We have been very satisfied

The human CHO-K1/M2/Gα15 cell line has significantly improved the consistency and reliability of our GPCR assays. The cells are easy to culture and have demonstrated excellent stability over multiple passages. The results we obtain are reproducible and accurate, making this cell line a top choice for GPCR research.

Excellent

The cell line's performance has exceeded our expectations

We've found the human CHO-K1/M2/Gα15 stable cell line to be extremely effective for our GPCR screening needs. The cell line provides a high signal-to-noise ratio, which is crucial for identifying potential drug candidates. The customer support provided was also very helpful in optimizing our assay conditions.

Excellent

It's a reliable choice for researchers

The human CHO-K1/M2/Gα15 cells are highly responsive and maintain their functionality across multiple experiments. We've observed consistent and reliable results, making this cell line a staple in our research toolkit.

Excellent

This cell line has been very effective for our GPCR assays

We appreciate the reliability and robustness of the human CHO-K1/M2/Gα15 stable cell line. It has streamlined our GPCR screening processes, and the cells consistently perform well in our assays. The quality of the cell line has greatly contributed to the success of our experiments.

Q&As

Can the human CHO-K1/M2/Gα15 cell line be used for high-throughput screening assays?

Yes, the human CHO-K1/M2/Gα15 cell line is well-suited for high-throughput screening assays. Its stable expression of the M2 receptor and Gα15 protein provides a robust and reliable platform for detecting GPCR activation across multiple samples. The cell line's consistent performance makes it an excellent choice for large-scale screening applications.

How often should the human CHO-K1/M2/Gα15 cell line be passaged?

The human CHO-K1/M2/Gα15 cell line should be passaged when it reaches 70-80% confluency to maintain optimal cell health and performance. Overgrowth can lead to reduced assay sensitivity and variability. Regular monitoring and timely passaging help maintain the cell line's stability and ensure reliable results in your experiments.

Can the human CHO-K1/M2/Gα15 cell line be used for long-term studies?

Yes, the human CHO-K1/M2/Gα15 cell line is suitable for long-term studies due to its stability and consistent performance. However, it is crucial to regularly monitor cell health, passage the cells appropriately, and ensure that they are maintained under optimal culture conditions to prevent drift in performance over time.