Product
Human CHO-K1/MT1/Gα15 Stable Cell Line
[CAT#: NCL20120088CR]
Melatonin MT1 Receptor Stable Cell Line
- Species:
- Human
- Applications:
- GPCR Screening
- Cell Types:
- CHO-K1; GPCR Cell
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Melatonin binds to two specific G-protein coupled receptors (GPCR), MT1 (MTNR1A/MEL1A) and MT2 (MTNR1B/MEL1B). MT1 receptors signal via inhibitory G proteins (Gαi and Gαo) leading to adenylate cyclase inhibition and possibly inositol phosphate stimulation in recombinant systems. In certain native tissues (e.g. sheep pars tuberalis, rat cerebral and caudal arteries) melatonin responses are presumably mediated through activation of MT1 receptors. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin.
Cell Types
CHO-K1; GPCR Cell
Applications
GPCR Screening
Research Areas
GPCR; Neural Stem Cell
Species
Human
Properties
Size
>1x10^6 Cells
Form
Frozen cells
Culture Medium
Ham's F12, 10% FBS, 200 μg/ml Zeocin, 100 μg/ml Hygromycin B
Freeze Medium
45% culture medium, 45% FBS, 10% DMSO
Receptor
MTNR1A
Family
Melatonin receptor
Strain
MT1/Gα15 Cell Line
Cell Purity
>95%
Shipping
Dry ice
Storage
Liquid nitrogen immediately upon delivery
Handling Advice
Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.
repeated exposure.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.
Target Details
Target
MTNR1A
Official Name
MTNR1A
Alternative Names
MT1 receptor; ML1A; MEL1A; ML1A; Mel1a; MEL-1A-R; mel1a melatonin receptor; mel1a receptor; melatonin receptor 1A; melatonin receptor type 1A; MelR
References
1. Mi, G., Liu, S., Zhang, J., Liang, H., Gao, Y., Li, N.,.. & Yang, Z. (2017). Levo-tetrahydroberberrubine produces anxiolytic-like effects in mice through the 5-HT 1A receptor. PloS one, 12(1), e0168964.
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
