Human MEF2A Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP298]

Human MEF2A knockout cell lysate

Species:: Human

Cell Types:: HeLa

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human MEF2A has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HeLa

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon8 and 1 bp insertion in exon8 and 5 bp deletion in exon8.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurogenesis

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

MEF2A

Official Name

MEF2A

Full Name

Myocyte Enhancer Factor 2A

Alternative Names

Myocyte Enhancer Factor 2A; Serum Response Factor-Like Protein 1; Mef2; MADS Box Transcription Enhancer Factor 2; Polypeptide A (Myocyte Enhancer Factor 2A); Myocyte-Specific Enhancer Factor 2A; ADCAD1; RSRFC4; RSRFC9;