NeuCELL™ VM Human Neural Progenitor Cell Line & Culture Media Kit
NeuCELL™ VM Kit contains human neural progenitor cells, optimized maintenance media & freezing media for culture of human neural stem cells.
- Cell Culture
- Cell Types:
- Neural Stem Cell; Neural Progenitor Cell
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NeuCELL™ VM Immortalized cells (NCL-2108-P002): NeuCELL™ VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. NeuCELL™ VM was derived from the ventral mesencephalon region of human fetal brain. Immortalized by retroviral transduction with the v-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the NeuCELL™ VM retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). NeuCELL™ VM can be differentiated in vitro to a high level of human dopaminergic neurons. Neurons differentiated from NeuCELL™ VM have furthermore been shown to be electophysiologically active. NeuCELL™ VM may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions.
NeuCELL™ NSC Maintenance Medium (NPC-M02): NeuCELL™ Neural Stem Cell (NSC) Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of NeuCELL™ immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of NeuCELL™ immortalized VM and CX neural stem cells. Withdrawal of the growth factors from NeuCELL™ NSC Maintenance Medium will result in the spontaneous differentiation of NeuCELL™ immortalized neural progenitor cells.
NeuCELL™ NSC Freezing Medium (NPC-M03): NeuCELL™ NSC Freezing Medium is qualified for use with NeuCELL™ immortalized human neural progenitor cell lines, CX (Catalog No. NCL-2108-P003) and VM (Catalog No. NCL-2108-P002) cultured in serum-free conditions with NeuCELL™ NSC Maintenance Medium (Catalog No. NPC-M02). The optimized formulation allows for consistent cryopreservation and high viability upon thawing and plating.
NeuCELL™ NSC Maintenance Medium (NPC-M02)
NeuCELL™ VM Immortalized Cells (NCL-2108-P002)