NeuroMab™ Anti-Amyloid Beta 1-40 BBB Shuttle Antibody(NRZP-1022-ZP3651)

Figure 1 Confirmation of the specificity of the 82E1 antibody by Western Blotting.

Antibody 82E1 does not react with amyloid beta (2-40) and amyloid beta (3-40), but only recognizes amyloid beta (1-40). On the other hand, it was confirmed that the 6E10 antibody does not react with amyloid β(1-40), but recognizes amyloid β(2-40) and amyloid β(3-40).

Figure 2 Confirmation of the specificity of the 82E1 antibody by Western Blotting

It was confirmed that the 82E1 monoclonal antibody does not react with APP, and only recognizes amyloid β and βCTF. On the other hand, it was confirmed that the 6E10 antibody recognized APP.

Figure 3 is the result of measuring the synthesis of β-amyloid (1-40) using a combination of 1A10 antibody and HRP-labeled 82E1 antibody.

Figure 4 shows the results of measuring the synthesis of β-amyloid (1-42) using a combination of 1C3 antibody and HRP-labeled 82E1 antibody.

Figure 5 β-amyloid precipitation inhibition test

The 82E1 antibody was diluted with PBS to a concentration of 1 mg/ml and injected intraperitoneally at a dose of 10 mg/Kg (oral) into 16-month-old Tg2576 genetically engineered pups expressing amyloid-β recursive protein (APP). rats (n = 3 body weight) once a week.

Figure 6 β-amyloid precipitation inhibition test

The 82E1 antibody was diluted with PBS to a concentration of 1 mg/ml and injected intraperitoneally at a dose of 10 mg/Kg (oral) into 16-month-old Tg2576 genetically engineered pups expressing amyloid-β recursive protein (APP). rats (n = 3 body weight) once a week.

Figure 7 Ten segments of the cortex and 5 segments of the hippocampus were selected and imaged for analysis using a microscopic digital camera and simple PCI software (Compix, Inc. Imaging Systems, USA). The deposition of amyloid β(1-40) and amyloid β(1-42) was expressed as the ratio (%) of the immunostained positive area in the whole area.

Figure 8 Amyloid β was extracted from the right side of the head (¼ of the brain) to measure amyloid β using assay kits for amyloid β(1-42) and human β-amyloid (1-42) ( x-42) in a sandwich ELISA system.