NeuroMab™ Anti-GABABR2 GABA-B receptor BBB Shuttle Antibody, Clone N81/37

Product Overview

Description

The BBB is a highly selective membrane that surrounds the blood vessels in the brain, and it can prevent many molecules, including potentially life-saving drugs, from entering the brain. The anti-GABBR2 BBB Shuttle Antibody is designed to bind to receptors on the surface of cells in the BBB, and once bound, the antibody can cross the BBB and into the brain. This technology has the potential to revolutionize the treatment of brain disorders by enabling drugs to reach the brain more effectively.

Immunogen

Fusion protein amino acids 862-913 (cytoplasmic C-terminus) of human GABABR2 (also known as Gamma-aminobutyric acid type B receptor subunit 2, GABA-B receptor 2, Gb2, G-protein coupled receptor 51 or GPR51, accession number O75899)
Mouse: 100% identity (52/52 amino acids identical)
Rat: 100% identity (52/52 amino acids identical)
<20% identity with GABABR1

Construction

We offer several options for BBB shuttle antibody design. Antibody construction can be customized according to customer requirements.

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Isotype

IgG1

Clone Number

N81/37

Applications

WB; IHC; ICC

Application Notes

Does not cross-react with GABABR1

Conjugation

Unconjugated

Product Properties

Research Use Only

For reseaech use only, not for human or dignostic use.

Target

GABBR2

Official Name

GABBR2

Full Name

GABA(B)R2

Alternative Names

Gamma-Aminobutyric Acid Type B Receptor Subunit 2; Gamma-Aminobutyric Acid (GABA) B Receptor; 2; G-Protein Coupled Receptor 51; GABA-B Receptor 2; GABA-B-R2; GABA-BR2; GABABR2; GPRC3B;

Uniprot ID

O75899

Product Pictures

Fig. 1 Adult rat brain membrane immunoblot.

Fig. 2 Immunoblot against membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N81/37 (left) or N52A/42 (right) TC supe.

Fig. 3 Immunoblot against brain membrane fractions from rat (RBM) and from GABABR2 knockout (KO) and wild-type (WT) mice probed with N81/37 (left) or N52A/42 (right) TC supe.

IHC

Fig. 4 Adult rat brain immunohistochemistry.

IEM

Fig. 5 Electron micrograph of N81/37 labelling in stratum radiatum of mouse hippocampal CA1 using a pre-embedding immunogold method. Immunoparticles are seen at postsynaptic sites (arrows) along the extrasynaptic plasma membrane of dendritic spines (s) and shafts of pyramidal cells forming synaptic contacts with excitatory axon terminals (b), and at presynaptic sites (arrowheads) in excitatory axon terminals (b).

Publications