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NeuroMab™ Anti-PlexinA1 BBB Shuttle Antibody,Clone NR3623P
- Host Species:
- Rabbit
- Species Reactivity:
- Mouse
- Applications:
- ELISA; In Vitro; Agonist
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.
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Fig.1 illustrates plots each showing the binding activity of anti-mouse PlexinA1 antibody to mouse PlexinA1.
The X-axis represents the antibody concentration, and the Y-axis represents the absorbance at a wavelength of 405 nm. The solid line represents binding to the Ba/F3 cell line expressing a high level of mouse PlexinA1, and the dashed line represents binding to Ba/F3 cells.
![](https://img.creative-biolabs.com/neuros/productImages/NRZP-1022-ZP2510-1.png)
Fig.2 illustrates plots each showing dose-dependent antagonistic activity of a control antibody or anti-PlexinA1 antibody (rabbit IgG) at concentrations of 0 to 100 μg/mL against semaphorin 3A-dependent mouse bone marrow-derived dendritic cell contraction.
Sema3A(+) represents values measured with the addition of 0.8 μg/mL of mouse semaphorin 3A, and Sema3A(−) represents values measured without the addition of mouse semaphorin 3A.
![](https://img.creative-biolabs.com/neuros/productImages/NRZP-1022-ZP2510-2.png)
Fig.3 illustrates a plot showing binding responses measured by immobilizing mouse chimeric antibodies hPANL#240-mFc, 359B-2-2-3-6-mFc, PXB693-mFc, PXB727-mFc, and PXB361b-mFc onto Protein G biosensors, and applying human PlexinA1 and mouse PlexinA1 thereto.
Sema3A(+) represents values measured with the addition of 0.8 μg/mL of mouse semaphorin 3A, and Sema3A(−) represents values measured without the addition of mouse semaphorin 3A.
![](https://img.creative-biolabs.com/neuros/productImages/NRZP-1022-ZP2510-3.png)
Fig.4 illustrates a plot showing binding affinities of the antagonist antibody mouse chimera PXB361b for mouse PlexinA1, mouse PleixinA2, and the chimeric protein of mouse PlexinA1 and PlexinA2. 1: Mouse PlexinA1 sema domain, 2: mouse PlexinA1/A2 sema domain chimera, 3: mouse PlexinA2 sema domain, Control Ab: control antibody, PXB361b: mouse chimeric antibody PXB361b-mFc.
Sema3A(+) represents values measured with the addition of 0.8 μg/mL of mouse semaphorin 3A, and Sema3A(−) represents values measured without the addition of mouse semaphorin 3A.
![](https://img.creative-biolabs.com/neuros/productImages/NRZP-1022-ZP2510-4.png)
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