NeuroMab™ Mouse Anti-Rat BSG Monoclonal Antibody (CBP1595)

Description

It recognizes a membrane glycoprotein of ~42-48 kDa, which is expressed at a high level on activated lymphocytes and at a low level on resting cells. CD147 is also present on some endothelial cells. The OX-47 antigen contains two Ig-like domains and an abnormal transmembrane domain with charged residues. CD147 is abundant on activated lymphocytes: it is a homolog of the chicken blood-brain barrier antigen HT7.

Immunogen

T blasts from a mixed lymphocyte reaction.

Species Reactivity

Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1595

Applications

FC; IF; IHC-Fr; WB

Application Notes

FCM (1/20); IF (1/20)

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1376

Form

Tissue Culture Supernatant - liquid

Formulation

Tissue Culture Supernatant-Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
0.09% Sodium Azide

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

CD147

Official Name

BSG

Full Name

Basigin

Alternative Names

BSG; 5F7; CD147; EMMPRIN; M6; OK; TCSF; basigin (Ok blood group); EMPRIN; SLC7A11

Gene ID

682 (Human); 12215 (Mouse)

Uniprot ID

P35613 (Human); P18572 (Mouse)

1. Paterson DJ et al. (1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. 2. Fossum, S. et al. (1991) The MRC OX-47 antigen is a member of the immunoglobulin superfamily with an unusual transmembrane sequence. 3. Cassella, J.P. et al. (1996) Ontogeny of four blood-brain barrier markers: an immunocytochemical comparison of pial and cerebral cortical microvessels. 4. Foda, H.D. et al. (2001) Ventilator-induced lung injury upregulates and activates gelatinases and EMMPRIN: attenuation by the synthetic matrix metalloproteinase inhibitor, Prinomastat (AG3340). 5. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations. 6. Betsuyaku, T. et al. (2003) Increased basigin in bleomycin-induced lung injury. 7. Du, Y. et al. (2008) Synthetic sandwich culture of 3D hepatocyte monolayer. 8. Nakao, Y. et al. (2011) Bile canaliculi formation by aligning rat primary hepatocytes in a microfluidic device. 9. Nugraha, B. et al. (2011) Galactosylated cellulosic sponge for multi-well drug safety testing. 10. Xia, L. et al. (2009) Laminar-flow immediate-overlay hepatocyte sandwich perfusion system for drug hepatotoxicity testing. 11. Zhang, S. et al. (2008) Microfabricated silicon nitride membranes for hepatocyte sandwich culture. 12. Cassella, J.P. et al. (1996) Ontogeny of four blood-brain barrier markers: an immunocytochemical comparison of pial and cerebral cortical microvessels. 13. Zhu, L. et al. (2016) A vertical-flow bioreactor array compacts hepatocytes for enhanced polarity and functions.