Mouse Anti-Human BSG Monoclonal Antibody (CBP1565)

Description

Human CD147 cell surface antigen, also known as Basigin, EMMPRIN and collagenase stimulating factor. CD147 is a single-pass type 1 transmembrane glycoprotein of 385 amino acids, with 3 potential N-glycosylation sites, a single N-terminal (distal) Ig-like C2-type domain and a more proximal Ig-like domain V-domain extracellular region. Produce mouse anti-human CD147 antibody, clone MEM-M6/1, and screen against the recombinant extracellular domain of huCD147 expressed in CHO cells and many other MEM-M6 clones. Epitope mapping shows that clone MEM-M6/1 binds to epitopes in the distal Ig-like C-2 type domain. This was confirmed by flow cytometry studies using construct-coated beads containing only distal Ig-like or proximal Ig-like domains.

Expression Host

CHO

Immunogen

The extracellular region of human CD147.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP1565

Applications

FC; WB

Research Areas

Growth and Development

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS

Preservatives

Preservative Free

Concentration

1 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

CD147

Official Name

BSG

Full Name

Basigin

Alternative Names

BSG; 5F7; CD147; EMMPRIN; M6; OK; TCSF; basigin (Ok blood group); EMPRIN; SLC7A11

Gene ID

682 (Human); 12215 (Mouse)

Uniprot ID

P35613 (Human); P18572 (Mouse)

1. Koch, C. et al. (1999) T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. 2. Nassif, X. et al. (2015) Cd147 as receptor for pilus-mediated adhesion of meningococci to vascular endothelia. 3. Bernard SC et al. (2014) Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonization. 4. Douglas, A.D. et al. (2014) Neutralization of Plasmodium falciparum merozoites by antibodies against PfRH5. 5. Crosnier C et al. (2011) Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum. 6. Wanaguru M et al. (2013) RH5-Basigin interaction plays a major role in the host tropism of Plasmodium falciparum. 7. Zenonos, Z.A. et al. (2015) Basigin is a druggable target for host-oriented antimalarial interventions. 8. Hahn, J.N. et al. (2015) The role of EMMPRIN in T cell biology and immunological diseases. 9. Jongruamklang, P. et al. (2018) Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of 36 blood group alleles among 396 Thai samples reveals region-specific variants.