NeuroMab™ Mouse Anti-CD80 Monoclonal Antibody (CBP2552)

Description

Mouse anti-human CD80 antibody, recognizes human CD80, also known as B7-1, a type 1 transmembrane protein of approximately 60 kDa, produced by macrophages and dendritic cells (Munro et al. 1994) And activated B cells ((Ranheim et al., 1993) CD80 is a member of the immunoglobulin superfamily, which has a single Ig-v-like domain, a single Ig-c-like domain, transmembrane sequence and short cytoplasmic structure The extracellular domain of the domain. Although the predicted molecular weight of human CD80 is ~33 kDa, there are multiple (8) potential N-glycosylation sites (Chen et al., 1998) that can cause migration of ~60 kDa. Human CD80 Together with CD86, it acts as a co-stimulatory molecule, and both are ligands of CD28 and CTLA-4 involved in T cell activation and proliferation (Azuma et al., 1993) (Vasu et al., 2003). Although CD80 and CD86 bind to the same receptor, Its affinity and binding kinetics show very different characteristics (van der Merwe et al., 1997).

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2552

Applications

FC

Application Notes

FC: 1/5-1/10

Relevant Diseases

Alzheimer's Disease; Neurodegenerative Disease

Research Areas

Neuroregeneration

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1861

Form

Liquid

Formulation

PBS, 0.09% Sodium Azide, 1% BSA

Preservatives

YES

Concentration

0.05 mg/ml

Purification

Protein A

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD80

Official Name

CD80

Full Name

CD80 Molecule

Alternative Names

CD80 Molecule; CD80 Antigen (CD28 Antigen Ligand 1, B7-1 Antigen); B-Lymphocyte Activation Antigen B7; CTLA-4 Counter-Receptor B7.1; Activation B7-1 Antigen; CD28LG1; CD28LG; LAB7; BB1;

Gene ID

941(Human); 12519(Mouse)

Uniprot ID

P33681(Human); Q00609(Mouse)

1. Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
2. Daubenberger, C.A. et al. (2007) Flow cytometric analysis on cross-reactivity of human-specific CD monoclonal antibodies with splenocytes of Aotus nancymaae, a non-human primate model for biomedical research.
3. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.