Rat Microglia, Neonatal

[CAT#: NCL-2103-P19]

Positive for F4/80 and CD11b/c

Species:: Rat

Cell Types:: Microglia

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Product Overview

Description

Rat microglia (RMcg) are extracted from the brains of normal rats on the first day after birth by standard methods. When resurrected under the recommended conditions, inoculated and cultured, RMcg forms an adherent culture.
Microglia originate from hematopoietic stem cells in the bone marrow and are resident macrophages in the central nervous system (CNS). Due to the presence of the blood-brain barrier, they are separated from the rest of the body. Microglia actively investigate the surrounding area and respond by removing damaged nerve cells, plaques and infectious agents. Since microglia have the function of immune response and maintenance of homeostasis in the central nervous system, they are related to neurodevelopment, central nervous system plasticity and repair, neuroinflammation, aging and neurodegeneration, neuropathic pain and infection.

Cell Types

Microglia

Species

Rat

Properties

Tissue Source

Normal, healthy Rat brain

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.