Mouse Microglia (SIM-A9), Immortalized

[CAT#: NCL-2105-P210-AM]

These cells can be used for disease modeling, drug screening and toxicology testing.

Species:: Mouse

Cell Types:: Microglia

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Product Overview

Description

Microglia are the resident macrophages of the brain and spinal cord. Small cells that are spontaneously immortal Murine microglia (SIM-A9) retain the ability to respond to exogenous inflammatory stimuli (LPS and β-amyloid), as well as expressing microglia-specific markers CD68 and Iba1.

Cell Types

Microglia

Species

Mouse

Properties

Freeze Medium

90% FBS+10% DMSO

Tissue Source

Brain

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

Negative

Sterility Testing

The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

Shipping

Frozen on dry ice

Storage

Liquid nitrogen immediately upon delivery

Research Use Only

For research use only

Quality Control

1) Perform functional tests, such as nitrate determination, to evaluate the production of nitric oxide; 2) ELISA method to measure TNFa secretion after inflammation stimulation; 3) Western blot and immunocytochemistry to confirm the specific markers of microglia after stimulation Such as the expression of CD68 and Iba1 and the expression of M1/M2 phenotype-related proteins (COX-2/iNOS and Arg-1); 4) A tail 1-42 listening or E. The uptake of E. coli-derived biological particles is used to determine the phagocytic activity of SIM-A9 cells.

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