Beta-2 Adrenergic Receptor Assay Cell Line

Description

Cell line information: cAMP- Maxensor ARDB2 Cell Line

Features

cAMP Maxsensor cells are cell lines that stably co-express label-free GPCR and cAMP Maxsensor biosensors to monitor the regulation of cAMP release on G protein. Each bottle of cAMP Maxsensor B2 adrenergic receptor (ADRB2) receptor cell line contains stable expression of the following Constructed U2OS cells:

(1) Unlabeled human B2 adrenergic receptor (ARDB2);

(2) cAMP Maxsensor biosensor (red fluorescence).

The cAMP Maxsensor B2 adrenergic receptor (ARDB2) cell line allows the analysis of compounds in the G protein signaling pathway through cAMP that involves receptor activation. When the agonist binds to ADRB2, it activates the G protein, which in turn triggers a cellular response mediated by cAMP. This leads to an increase in the red fluorescence intensity of the cAMP biosensor. Therefore, the activity of living cells can be easily quantified in a dose-response manner through the fluorescence emission in the microplate reader.

Cell Types

Other Cells

Application Notes

About Red cAMP Maxensor Biosensor:
The red cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before stimulating cAMP production, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the red cAMP Maxensor biosensor, which promotes the relocation of cells in the transport of cell vesicles. In cell lines that co-express the red cAMP Maxensor biosensor and the GPCR of interest, the activity of living cells can be easily quantified by fluorescence granularity image analysis or fluorescence intensity analysis.

Research Areas

GPCR

Assay

Beta-2 Adrenergic Receptor Assay

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

No bacteria, yeast, fungi, mycoplasma, virus

Target

ADRB2

Official Name

ADRB2

Full Name

Beta2 Adrenoceptor

Alternative Names

ADRB2; ADRB2R; ADRBR; B2AR; BAR; BETA2AR; adrenoceptor beta 2