Product
Beta-2 Adrenergic Receptor Assay Cell Line
[CAT#: NCL2110P331]
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Cell line information: cAMP- Maxensor ARDB2 Cell Line
Features
cAMP Maxsensor cells are cell lines that stably co-express label-free GPCR and cAMP Maxsensor biosensors to monitor the regulation of cAMP release on G protein. Each bottle of cAMP Maxsensor B2 adrenergic receptor (ADRB2) receptor cell line contains stable expression of the following Constructed U2OS cells:
(1) Unlabeled human B2 adrenergic receptor (ARDB2);
(2) cAMP Maxsensor biosensor (red fluorescence).
The cAMP Maxsensor B2 adrenergic receptor (ARDB2) cell line allows the analysis of compounds in the G protein signaling pathway through cAMP that involves receptor activation. When the agonist binds to ADRB2, it activates the G protein, which in turn triggers a cellular response mediated by cAMP. This leads to an increase in the red fluorescence intensity of the cAMP biosensor. Therefore, the activity of living cells can be easily quantified in a dose-response manner through the fluorescence emission in the microplate reader.
(1) Unlabeled human B2 adrenergic receptor (ARDB2);
(2) cAMP Maxsensor biosensor (red fluorescence).
The cAMP Maxsensor B2 adrenergic receptor (ARDB2) cell line allows the analysis of compounds in the G protein signaling pathway through cAMP that involves receptor activation. When the agonist binds to ADRB2, it activates the G protein, which in turn triggers a cellular response mediated by cAMP. This leads to an increase in the red fluorescence intensity of the cAMP biosensor. Therefore, the activity of living cells can be easily quantified in a dose-response manner through the fluorescence emission in the microplate reader.
Application Notes
About Red cAMP Maxensor Biosensor:
The red cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before stimulating cAMP production, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the red cAMP Maxensor biosensor, which promotes the relocation of cells in the transport of cell vesicles. In cell lines that co-express the red cAMP Maxensor biosensor and the GPCR of interest, the activity of living cells can be easily quantified by fluorescence granularity image analysis or fluorescence intensity analysis.
The red cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before stimulating cAMP production, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the red cAMP Maxensor biosensor, which promotes the relocation of cells in the transport of cell vesicles. In cell lines that co-express the red cAMP Maxensor biosensor and the GPCR of interest, the activity of living cells can be easily quantified by fluorescence granularity image analysis or fluorescence intensity analysis.
Research Areas
GPCR
Assay
Beta-2 Adrenergic Receptor Assay
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Target Details
Target
ADRB2
Official Name
ADRB2
Full Name
Beta2 Adrenoceptor
Alternative Names
ADRB2; ADRB2R; ADRBR; B2AR; BAR; BETA2AR; adrenoceptor beta 2
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
