Human CHO-K1/Gqi5 Stable Cell Line

[CAT#: NCL20120142CR]

Gqi5 Stable Cell Line

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

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Product Overview

Description

It is a CHO-K1 cell line stably expressing the chimeric Gqi5 alpha subunit protein which a chimeric Gq protein instead of the last five carboxyl-terminal amino acids from Gi. It is used as a host cell for transfection expression of Gi/o-coupled receptors, the constitutively expressed Gqi5 protein in the cells allows many transfected receptors which normally inhibit the cAMP pathway, to couple to Gq signal transduction and mobilize intracellular calcium. The cell line carries the hygromycin B resistance gene and is resistant to hygromycin B.

Cell Types

Other Cells

Applications

GPCR Screening

Research Areas

GPCR

Species

Human

Properties

Size

>1x10^6 Cells

Form

Frozen cells

Culture Medium

Ham's F12, 10% FBS, 100 μg/ml Hygromycin B

Freeze Medium

45% culture medium, 45% FBS, 10% DMSO

Family

Control

Strain

Gqi577 Cell Line

Cell Purity

>95%

Shipping

Dry ice

Storage

Liquid nitrogen immediately upon delivery

Handling Advice

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or
repeated exposure.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents.

Target Details

Target

GNA15

Official Name

GNA15

Alternative Names

Gqi5 alpha subunit protein; Gqi5

References

1. Tomabechi, Y., Krippner, G., Rendle, P. M., Squire, M. A., & Fairbanks, A. J. (2013). Glycosylation of pramlintide: synthetic glycopeptides that display in vitro and in vivo activities as amylin receptor agonists. Chemistry-A European Journal, 19(45), 15084-15088.

Publications