Product

Human HTR3D Knockout A549 Cell Lysate

[CAT#: NCL2008ZP382]

Human HTR3D knockout cell lysate

Species:: Human

Cell Types:: A549 Cell

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

This is a A549 cell transfected lysate in which Human HTR3D has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

A549 Cell

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon6 and 2 bp insertion in exon6.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neurotransmission

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X,Y
D5S818: 11
D13S317: 11
D7S820: 8, 11
D16S539: 11, 12
vWA: 14
TH01: 8,9.3
TPOX: 8,11
CSF1PO: 10, 12

Tissue Source

Lung

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

HTR3D

Official Name

HTR3D

Full Name

5-Hydroxytryptamine Receptor 3D

Alternative Names

5-Hydroxytryptamine Receptor 3D; 5-Hydroxytryptamine (Serotonin) Receptor 3 Family Member D; 5-Hydroxytryptamine (Serotonin) Receptor 3D; Ionotropic; Serotonin Receptor 3D; 5-Hydroxytryptamine Receptor 3 Subunit D; Serotonin 5-HT-3D Receptor; 5-HT3-D; 5-HT3D; 5HT3D;

Publications