MC3 Melanocortin Receptor Assay Cell Line

[CAT#: NCL2110P282]

Cell Types:: Other Cells

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Product Overview

Description

Cell line information: MCR3 Maxensor cell line
Official Full Name: Melanocortin receptor 3
DNA Acc. Number: AY227893
Host Cell: U2OS
Resistance: G418 + Puromycin
Storage: Liquid Nitrogen

Features

The cAMP Maxensor MCR3 contains U2OS cells stably expressing cAMP Maxensor biosensor and melanocortin receptor 3. The Creative Biolabs cAMP Maxensor MCR3 cell line is designed to test compounds or analyze their ability to modulate melanocortin receptor 3 (MCR3). When the agonist binds to MCR3, it activates the G protein, which in turn triggers a cellular response mediated by cAMP. This cell line has been verified by analyzing the intracellular distribution of cAMP Maxensor biosensor by measuring the increase of cAMP in the cytosol. This cell line allows image analysis of compound-induced stimuli. In high content analysis (HCA), the use of human (Nle4, D-Phe7)-melanocyte stimulating hormone (MSH) as an agonist validated this highly reproducible assay.

Cell Types

Other Cells

Application Notes

About cAMP Maxensor Biosensor
The cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before cAMP stimulates, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the folding of the Maxensor biosensor, which promotes the relocation of the cells in the vesicle transport of the cells. In cell lines co-expressing Maxensor biosensor and GPCR, after activating the GPCR with the agonist of interest, the viability of living cells can be easily quantified by image analysis of changes in cell density.

Research Areas

GPCR

Assay

MC3 Melanocortin Receptor Assay

Assay Description

Creative Biolabs' MC3 Melanocortin Receptor Assay uses Maxensor Biosensors technology to measure MC3R receptor activation. MC3 melanocortin receptor signaling occurs through adenylate cyclase stimulation through the interaction of trimerized Gs protein, increased cAMP, and downstream L-type calcium channels. In this assay, we use the cAMP-Maxensor biosensor to measure receptor activation/inhibition through cAMP accumulation. The high reproducibility of this assay allows monitoring of MC3 melanocortin receptor activation in high-throughput screening.

Assay Characteristics

Readout: cAMP Flux (Gs activation)

Agonist: (Nle4,D-Phe7)-a-MSH

EC50 Agonist: 3.96 x 10⁶-17 M for cAMP Flux

Type of Assay: Cell-Based/Functional

Detection Method: Fluorimetry

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.