Mouse bEnd.3 [BEND3] Cells

[CAT#: NCB2108FY0001]

Animal cells

Species:: Mouse

Applications:: 3D Cell Culture

Cell Types:: BBB-related Cells

Cell Subtypes:: Brain Endothelial Cells

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Request Pricing

Inquiry

Product Overview

Description

Incubate the culture at 37°C in a suitable incubator. A 95% Air, 5% CO2 in air atmosphere is recommended if using the medium described on this product.

Cell Types

BBB-related Cells

Cell Subtypes

Brain Endothelial Cells

Applications

3D Cell Culture

Application Notes

The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen.
The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).

Relevant Diseases

Endothelioma

Research Areas

Neural Cell

Species

Mouse

Expression Profile

von Willebrand factor-; ICAM-1+; VCAM-1+; MAdCAM-1+ ; The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd.

Properties

Form

Frozen

Growth Pattern

Adherent

Morphology

Endothelial

Immortalization Method

Polyoma middle T antigen

Culture Medium

The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Tissue Source

Brain; Cerebral cortex

Age

6 weeks

Strain

BALB/c

Shipping

Dry ice

Storage

Vapor phase of liquid nitrogen

Handling Advice

Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.