Mouse bEnd.3 [BEND3] Cells
[CAT#: NCB2108FY0001]
Animal cells
- Species:
- Mouse
- Applications:
- 3D Cell Culture
- Cell Types:
- BBB-related Cells
- Cell Subtypes:
- Brain Endothelial Cells
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Product Overview
Description
Incubate the culture at 37°C in a suitable incubator. A 95% Air, 5% CO2 in air atmosphere is recommended if using the medium described on this product.
Cell Types
BBB-related Cells
Cell Subtypes
Brain Endothelial Cells
Applications
3D Cell Culture
Application Notes
The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen.
The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).
The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).
Relevant Diseases
Endothelioma
Research Areas
Neural Cell
Species
Mouse
Expression Profile
von Willebrand factor-; ICAM-1+; VCAM-1+; MAdCAM-1+ ; The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd.
Properties
Form
Frozen
Growth Pattern
Adherent
Morphology
Endothelial
Immortalization Method
Polyoma middle T antigen
Culture Medium
The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Tissue Source
Brain; Cerebral cortex
Age
6 weeks
Strain
BALB/c
Shipping
Dry ice
Storage
Vapor phase of liquid nitrogen
Handling Advice
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
Research Use Only
For research use only
Donor Information
Blood Type
ICAM-1 +; VCAM-1 +; MAdCAM-1 +
Additional Donor Information
Transformed cells were infected by NTKmT retroviral vector expressing polyoma virus intermediate T antigen.
Publications
Publications (0)
Neural Cell Culture
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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