Product
Human Brain Microvascular Pericytes (HBMVPCs)
[CAT#: NCL-2103-P229]
Q&As(3)
- Species:
- Human
- Cell Types:
- BBB-related Cells
- Cell Subtypes:
- Pericytes
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Human brain microvascular pericytes play an important role in the formation and function of the blood-brain barrier, the regulation of angiogenesis, the survival of endothelial cells and the phagocytosis of cell debris.
The human capillaries are isolated from normal human cerebral cortex tissue. Purromycin-resistant hbmvp is shipped in vials with dry ice in the third passage. It is recommended to use pericyte culture medium containing 5% fetal bovine serum and growth supplement (NCL-2103-P234). The average number of cells doubled in culture>12.
The human capillaries are isolated from normal human cerebral cortex tissue. Purromycin-resistant hbmvp is shipped in vials with dry ice in the third passage. It is recommended to use pericyte culture medium containing 5% fetal bovine serum and growth supplement (NCL-2103-P234). The average number of cells doubled in culture>12.
Cell Types
BBB-related Cells
Cell Subtypes
Pericytes
Species
Human
Properties
Size
500,000+ Cells - Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Frozen (Dry Ice/Liquid Nitrogen)
Storage
Keep frozen in liquid nitrogen until plating
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Donor Information
Donor Health
Normal
Publications
Publications (0)
Neural Cell Lines
Customer Reviews and Q&As
Q&As
What's the appropriate culture medium for Brain Microvascular Pericytes (HBMVPCs)?
Pericyte-Growth Media, Cat.#NCL-2103-P234
Can these cells be used for 3D BBB model establish?
Yes, you can purchase these cells together with Human Brain Microvascular Endothelial Cells, Cat.#NCL-2103-P133, and Human Endothelial-Growth Media, Cat.# NCL-2103-P178.
Do you provide ready-to-use 3B human BBB model?
We offer a 3D Human Blood Brain Barrier Model [CAT#: NCL-2103-P187], which can be customized according to your requirements. Please note that the preparation of this model will take approximately 4 weeks.
For Research Use Only. Not For Clinical Use.
