Human Brain Pericytes, GFP Expressed

[CAT#: NCL-2103-P228]

Species:: Human

Cell Types:: BBB-related Cells

Cell Subtypes:: Pericytes

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Product Overview

Description

Human brain microvascular pericytes expressing GFP were isolated from normal human brain tissue and transfected with GFP lentiviral particles. Puromycin is resistant, and GFP-HBMVPCs are shipped in frozen vials (available proliferating culture with a confluency of>90 % upon request) (cells are provide at passage 5). It is recommended to use pericyte growth medium (CAT# NCL-2103-P234) containing 5% fetal bovine serum and growth supplements for culture. The cells have an average number doubling level>10 when cultured.

Cell Types

BBB-related Cells

Cell Subtypes

Pericytes

Species

Human

Properties

Size

500,000 cells - Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Frozen (Dry Ice/Liquid Nitrogen)

Storage

Keep frozen in liquid nitrogen until plating

Handling Advice

Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Donor Information