Product
Neural Stem Cell Freezing Medium (1X) (Human&Mouse)
[CAT#: NCC200532ZP]
Optimized & validated for Stem cell culture.
- Applications:
- Cell Cryopreservation; Stem Cell Culture
- Cell Types:
- Neural Stem Cell; Neural Progenitor Cell
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Cell Types
Neural Stem Cell; Neural Progenitor Cell
Species Reactivity
Human; Mouse
Applications
Cell Cryopreservation; Stem Cell Culture
Research Areas
Stem Cell Research
Properties
Formulation
Contains 10% DMSO
Form
Liquid
Serum Level
Serum-Free
Shipping
Dry ice
Storage
Store at -20°C.
Handling Advices
1. Thaw the cell culture freezing medium completely and gently rotate the bottle to mix thoroughly. During use, place the freezing medium on ice.
2. The cells to be frozen should be in the late logarithmic growth phase.
3. The single layer will need to dissociate. After dissociation, the cells were resuspended in neural stem cell basal medium and counted to determine viability and number.
4. Centrifuge the cells at 300 x g for 3 minutes. Remove the medium above the pellet.
5. Resuspend the cells in neural stem cell freezing medium at a concentration of approximately 4 x 106 cells / mL. Freeze 1 mL cells / vial. After the cells are resuspended and aliquoted into appropriate cryogenic storage flasks, they can be placed in a freezing container and the normal freezing procedure should begin within five minutes.
6. Cells must be stored at -80 ° C or below. For long-term storage, cells should be stored in an ultra-low temperature refrigerator (-150 ° C) or liquid nitrogen (-196 ° C).
2. The cells to be frozen should be in the late logarithmic growth phase.
3. The single layer will need to dissociate. After dissociation, the cells were resuspended in neural stem cell basal medium and counted to determine viability and number.
4. Centrifuge the cells at 300 x g for 3 minutes. Remove the medium above the pellet.
5. Resuspend the cells in neural stem cell freezing medium at a concentration of approximately 4 x 106 cells / mL. Freeze 1 mL cells / vial. After the cells are resuspended and aliquoted into appropriate cryogenic storage flasks, they can be placed in a freezing container and the normal freezing procedure should begin within five minutes.
6. Cells must be stored at -80 ° C or below. For long-term storage, cells should be stored in an ultra-low temperature refrigerator (-150 ° C) or liquid nitrogen (-196 ° C).
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Publications
Publications (0)
Neural Cell Culture
For Research Use Only. Not For Clinical Use.
